The Balance of HCO3- Secretion vs. Reabsorption in the Endometrial Epithelium Regulates Uterine Fluid pH
| Autor: | Yi-Min Guo, Li-Ming Chen, Jichun Yang, Zhang-Dong Xie, Tong-Hui Xu, Shao-Fang Wang, Ying Liu, Mei-Juan Ren |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
inorganic chemicals medicine.medical_specialty endocrine system transepithelial ion transport Physiology Uterus HCO-3 reabsorption lcsh:Physiology 03 medical and health sciences 0302 clinical medicine Capacitation Physiology (medical) Internal medicine endometrial epithelium medicine SLC26A6 ion reabsorption reproductive and urinary physiology Original Research Estrous cycle 030219 obstetrics & reproductive medicine HCO-3 transporter lcsh:QP1-981 biology Chemistry Reabsorption urogenital system Apical membrane 030104 developmental biology Endocrinology medicine.anatomical_structure pH regulation ion secretion biology.protein Cotransporter Homeostasis hormones hormone substitutes and hormone antagonists |
| Zdroj: | Frontiers in Physiology Frontiers in Physiology, Vol 9 (2018) |
| ISSN: | 1664-042X |
| Popis: | Uterine fluid contains a high concentration of [Formula: see text] which plays an essential role in sperm capacitation and fertilization. In addition, the [Formula: see text] concentration in uterine fluid changes periodically during the estrous cycle. It is well-known that the endometrial epithelium contains machineries involving the apical SLC26 family anion exchangers for secreting [Formula: see text] into the uterine fluid. In the present study, we find for the first time that the electroneutral Na+/[Formula: see text] cotransporter NBCn1 is expressed at the apical membrane of the endometrial epithelium. The protein abundance of the apical NBCn1 and that of the apical SLC26A4 and SLC26A6 are reciprocally regulated during the estrous cycle in the uterus. NBCn1 is most abundant at diestrus, whereas SLC26A4/A6 are most abundant at proestrus/estrus. In the ovariectomized mice, the expression of uterine NBCn1 is inhibited by β-estradiol, but stimulated by progesterone, whereas that of uterine SLC26A4/A6 is stimulated by β-estradiol. In vivo perfusion studies show that the endometrial epithelium is capable of both secreting and reabsorbing [Formula: see text]. Moreover, the activity for [Formula: see text] secretion by the endometrial epithelium is significantly higher at estrus than it is at diestrus. The opposite is true for [Formula: see text] reabsorption. We conclude that the endometrial epithelium simultaneously contains the activity for [Formula: see text] secretion involving the apical SLC26A4/A6 and the activity for [Formula: see text] reabsorption involving the apical NBCn1, and that the acid-base homeostasis in the uterine fluid is regulated by the finely-tuned balance of the two activities. |
| Databáze: | OpenAIRE |
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