Relative levels of dietary EPA and DHA impact gastric oxidation and essential fatty acid uptake
| Autor: | Judith Storch, Matthew Boller, Gabriel Dasilva, Isabel Medina |
|---|---|
| Přispěvatelé: | Ministerio de Economía y Competitividad (España) |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Docosahexaenoic Acids Bioavailability Endocrinology Diabetes and Metabolism Lipid peroxidation Clinical Biochemistry Biological Availability Bioaccessibility Biochemistry Article Intestinal absorption 03 medical and health sciences chemistry.chemical_compound Fish Oils Essential fatty acid Lipid oxidation Humans Food science Caco-2 cells Molecular Biology chemistry.chemical_classification 030109 nutrition & dietetics Nutrition and Dietetics Fatty Acids Essential Stomach food and beverages Metabolism TIM Eicosapentaenoic acid Diet Soybean Oil 030104 developmental biology Eicosapentaenoic Acid chemistry Docosahexaenoic acid Dietary Supplements ω-3 PUFA Digestion lipids (amino acids peptides and proteins) Caco-2 Cells Oxidation-Reduction Polyunsaturated fatty acid |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0955-2863 |
| DOI: | 10.1016/j.jnutbio.2017.11.007 |
| Popis: | 8 pages, 4 figures, 1 table Previous research showed that increasing the proportion of docosahexaenoic acid (DHA) in marine lipid supplements significantly reduces associated health benefits compared with balanced eicosapentaenoic acid (EPA):DHA supplementation Dasilva et al., 2015 [1]. It was therefore hypothesized that the EPA and DHA molecules might have differential resistance to oxidation during gastric digestion and that the oxidation level achieved could be inversely correlated with intestinal absorption and, hence, with the resultant health benefits. Accordingly, we tested this proposed mechanism of action by investigating the degree of oxidation in the stomach, and the levels of bioaccessible lipids, of varying molar proportions of DHA and EPA (2:1, 1:1 and 1:2) using the dynamic gastrointestinal tract model TIM-1. In addition, small intestine enterocyte absorption and metabolism were simulated by Caco-2 cell monolayers that were incubated with these same varying proportions of DHA and EPA, and comparing oxidized and nonoxidized polyunsaturated fatty acids (PUFAs). The results show an inverse correlation between lipid oxidation products in the stomach and the levels of bioaccessible lipids. The balanced 1:1 EPA:DHA diet resulted in lower oxidation of PUFAs during stomach digestion relative to the other ratios tested. Finally, cell-based studies showed significantly lower assimilation of oxidized EPA and DHA substrates compared to nonoxidized PUFAs, as well as significant differences between the net uptake of EPA and DHA. Overall, the present work suggests that the correct design of diets and/or supplements containing marine lipids can strongly influence the stability and bioaccessibility of PUFAs during gastrointestinal digestion and subsequent absorption. This could modulate their health benefits related with inflammation, oxidative stress and metabolic disorders This work was supported by the Spanish Ministerio de Economía y Competitividad (AGL2013-49079-C2-1-R) and the National Institutes of Health (R01-DK38389). The Consejo Superior de Investigaciones Científicas and the University of Santiago de Compostela are gratefully acknowledged for the doctoral fellowship to Gabriel Dasilva. Xunta de Galicia and Axencia Galega de Innovación are also thankfully recognized for the financial support to Gabriel Dasilva |
| Databáze: | OpenAIRE |
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