Pitfalls in the Preparation of Monotypic Agglutinating Antisera for Bordetella Pertussis
| Autor: | L. B. Holt |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
Microbiology (medical)
Bordetella pertussis Whooping Cough Microbiology Antibodies Serology Mice Agglutinin Antigen Antibody Specificity Immunity Agglutination Tests Animals Serotyping Pertussis Vaccine Antiserum biology Immune Sera Immunochemistry General Medicine biology.organism_classification Virology Vaccination biology.protein Rabbits Antibody |
| Zdroj: | Journal of Medical Microbiology. 1:169-180 |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/00222615-1-2-169 |
| Popis: | Summary Agglutination tests showed that 5 cultures of Bordetella pertussis, strain no. 18/323, which are used in Britain to assess the protective value of whoopingcough vaccine by mouse-protection tests, are not serologically identical. “Monotypic” antisera used for the typing of Bord. pertussis by slideagglutination were found not to be serologically “pure”. A number of sera supposed to contain antibodies for factor 1 only proved to contain antibodies for a variety of other antigens, including factors 2 and 3. Several cultures in which only factor 1 was detected by slide agglutination were used as vaccines in rabbits. The resulting antisera contained other antibodies, and in some cases large amounts of antibody for factor 2 were detected. Individual rabbits varied considerably in the relative amounts of agglutinin for factors 1, 2 and 3 produced as a result of vaccination with the same bacterial suspension. The implications of these findings were examined in relation to the claim that immunity to whooping-cough is influenced by the serological type of the vaccine strain of Bord. pertussis. |
| Databáze: | OpenAIRE |
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