Involvement of RNase 7 in the malignant potential of oral squamous cell carcinoma cells
| Autor: | Puja Neopane, Jun Sato, Tetsuro Morikawa, Masato Saitoh, Koki Yoshida, Durga Paudel, Yoshihito Kurashige, Osamu Uehara, Yoshihiro Abiko |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Cancer Research
Carcinogenesis RNase P Cell Biology Ribonucleases Cell Movement Cell Line Tumor Biomarkers Tumor medicine Humans Neoplasm Invasiveness RNA Small Interfering Cell Proliferation Gene knockdown Oncogene Squamous Cell Carcinoma of Head and Neck Mouth Mucosa Cell Differentiation General Medicine Cell cycle Immunohistochemistry Immunity Innate Gene Expression Regulation Neoplastic stomatognathic diseases HaCaT medicine.anatomical_structure Oncology Cell culture Gene Knockdown Techniques Cancer research Mouth Neoplasms Keratinocyte |
| Zdroj: | Oncology Reports. 44:1216-1223 |
| ISSN: | 1791-2431 1021-335X |
| DOI: | 10.3892/or.2020.7678 |
| Popis: | RNase 7 is involved in the innate immunity of the oral epithelium. Variations in the expression levels of RNase 7 have been reported in cutaneous squamous cell carcinoma, but not in oral squamous cell carcinoma (OSCC). The present study investigated the expression levels of RNase 7 in OSCC and its role in the malignant potential of these cells. The localization of RNase 7 in OSCC tissue sections was determined via immunohistochemistry. Positive staining for RNase 7 was observed around the epithelial pearls and spinous cells of the OSCC tissues. Four different types of OSCC cell lines (OSC‑19, BSC‑OF, SAS, and HSC‑2) and a normal keratinocyte (HaCaT) were used. The mRNA and protein expression levels of RNase 7 were significantly higher in the OSCC cells compared to the HaCaT cells. Based on our hypothesis that high levels of RNase 7 expression may be involved in the malignant potential of OSCC cells, the effect of RNase 7 knockdown on both proliferation and invasion were evaluated by transfecting the cells with siRNA. Cell numbers, cell invasion, and MMP 9 expression levels were significantly higher in the siRNA‑BSC‑OF, ‑SAS, and ‑HSC‑2 cells compared to the BSC‑OF, SAS, and HSC‑2 cells. The extent of differentiation of the siRNA‑OSCC cells was examined using the differentiation and undifferentiation markers involucrin (INV) and K14, respectively. The expression level of K14 was significantly higher in the siRNA‑OSCC cells compared to the OSCC cells. Alternatively, HSC‑2 and SAS cells demonstrated higher expression levels of INV compared to the siRNA‑HSC‑2 and ‑SAS cells. These findings indicate that RNase 7 may contribute to the suppression of the malignant potential of OSCC. |
| Databáze: | OpenAIRE |
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