Frog (Pelophylax bergeri, Günther 1986) endocrine disruption assessment: characterization and role of skin poly(ADP-ribose) polymerases

Autor: Fagr Kh. Abdel-Gawad, Giulia Guerriero, Sofiane Labar, Maria Violetta Brundo, Anna De Maio, D. Rabbito, Samantha Trocchia, Giancarlo Palumbo, Anna Rita Bianchi
Přispěvatelé: Guerriero, Giulia, Brundo, Maria Violetta, Labar, Sofiane, Bianchi, Anna Rita, Trocchia, Samantha, Rabbito, Dea, Palumbo, Giancarlo, Abdel-Gawad, Fagr Kh., de Maio, Anna
Rok vydání: 2016
Předmět:
0301 basic medicine
Amphibian
Gene isoform
Male
medicine.medical_specialty
Cytoplasm
Ranidae
DNA damage
Poly ADP ribose polymerase
Health
Toxicology and Mutagenesis
Poly(ADP-ribose)
Endocrine disruption assessment
Pelophylax bergeri
010501 environmental sciences
Endocrine Disruptors
01 natural sciences
Frog skin
03 medical and health sciences
biology.animal
Internal medicine
Testis
medicine
Environmental Chemistry
Animals
Humans
0105 earth and related environmental sciences
Skin
Cell Nucleus
biology
Poly(ADP-ribose) polymerase
General Medicine
Metals bioaccumulation
biology.organism_classification
Molecular biology
Pollution
Androgen receptor
030104 developmental biology
Endocrinology
Poikilotherm
Italy
Amphibian population decline
Poly(ADP-ribose) Polymerases
Frog Skin
Androgen receptor expression
Water Pollutants
Chemical
DNA Damage
Environmental Monitoring
Zdroj: Environmental science and pollution research international. 25(19)
ISSN: 1614-7499
Popis: Model of the our research was the adult male amphibian anura, Pelophylax bergeri, poikilotherm species not considered threatened by the IUCN, sampled in representative sites at different degree. In the first phase, a biochemical characterization of the ADP-ribosylation on the skin of barcoded amphibian anura collected from Matese Lake (clean reference site in CE, Italy) was carried out. Two PARP isoforms were evidence: the first of 66 kDa is localized into nucleus and activated by DNA damage; the second of 150 kDa is in cytoplasm, as demonstrated by biochemical and immunohistochemical analysis. Subsequently, the PARP activity, the quantitative expression of androgen receptor gene, and the levels of arsenic and chromium in skin and testis of frog and soil, water, and sediment collected from sites at different degrees of pollution were measured. A significant variation of PARP activity and androgen receptor expression levels was detected in both tissues of barcoded frogs from Sarno and Scafati, along Sarno River (SA, Italy), suggesting that a PARP activation is correlated to pollution and to steroid-regulated physiology disruption.
Databáze: OpenAIRE
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