Persistent production of an integrase-deleted HIV-1 variant with no resistance mutation and wild-type proviral DNA in a treated patient
| Autor: | Christophe Ramière, Patrice Andre, Fabienne Venet, Mary-Anne Trabaud, Laurent Cotte, Corinne Ronfort, Guillaume Monneret, Jean-Claude Tardy, J. Saison |
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| Přispěvatelé: | Infections Virales et Pathologie Comparée - UMR 754 (IVPC), Institut National de la Recherche Agronomique (INRA)-École pratique des hautes études (EPHE)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Rétrovirus et Pathologie Comparée (RPC), Institut National de la Recherche Agronomique (INRA)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL), Hospices Civils de Lyon [D50624], École pratique des hautes études (EPHE), Université de Lyon-Université de Lyon-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Viral/genetics
[SDV]Life Sciences [q-bio] Mutant HIV Infections Defective virus Lopinavir Proviruses Leukocytes Polymerase Sequence Deletion 0303 health sciences biology Defective Viruses Viral Load Resistance mutation Mononuclear/virology 3. Good health Integrase Infectious Diseases Lamivudine Combination Drug Therapy Combination Zidovudine Sequence Analysis Anti-HIV Agents HIV Infections/*drug therapy/genetics Zidovudine/therapeutic use Immunology Molecular Sequence Data Defective Viruses/genetics/*pathogenicity Integrases/*genetics HIV-1/*genetics Virus 03 medical and health sciences Drug Therapy Virology Humans Lamivudine/therapeutic use Gene Proviruses/genetics 030304 developmental biology Immune Evasion Lopinavir/therapeutic use Immune Evasion/genetics Integrases Base Sequence 030306 microbiology Wild type Sequence Analysis DNA DNA Molecular biology Viral Load/drug effects DNA Viral biology.protein HIV-1 Leukocytes Mononuclear Anti-HIV Agents/*therapeutic use |
| Zdroj: | AIDS RESEARCH AND HUMAN RETROVIRUSES AIDS RESEARCH AND HUMAN RETROVIRUSES, 2015, 31 (1), pp.142-9. ⟨10.1089/AID.2014.0129⟩ AIDS Research and Human Retroviruses AIDS Research and Human Retroviruses, Mary Ann Liebert, 2015, 31 (1), pp.142-149. ⟨10.1089/AID.2014.0129⟩ |
| ISSN: | 0889-2229 |
| DOI: | 10.1089/AID.2014.0129⟩ |
| Popis: | Supplementary Data; International audience; An HIV-infected patient presenting an unexpected viral escape under combined antiretroviral treatment is described. The virus isolated from plasma contained a large deletion in the HIV-1 integrase gene but no known resistance mutation. Nested polymerase chain reactions (PCRs) with patient virus integrase-specific primers and probes were developed and used to detect the mutant from plasma, blood, rectal biopsies, and sperm. The variant progressively emerged during a period of therapy-induced virosuppression, and persisted at a low but detectable level for at least 5 years. Surprisingly, proviral DNA from lymphocytes, rectal cells, and sperm cells was, and remained, mainly wild type. Cellular HIV RNA with the deletion was detected only once from the rectum. The origin and mechanisms underlying this so far not described production at a detectable level are largely hypothetical. This observation raised concern about the ability of defective viruses to spread. |
| Databáze: | OpenAIRE |
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