Aptamer BC 007 - Efficient binder of spreading-crucial SARS-CoV-2 proteins
| Autor: | Susanne Becker, Hardy Weisshoff, Oxana Krylova, Hans-Dirk Düngen, Johannes Müller, Peter Göttel, André Dallmann, Annekathrin Haberland, Heike Nikolenko |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Aptamer Re-purposing Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA-dependent RNA polymerase medicine.disease_cause Clinical research 03 medical and health sciences 0302 clinical medicine medicine BC 007 Pharmaceutical sciences Antiviral lcsh:Social sciences (General) lcsh:Science (General) Coronavirus Pharmacology Infectious disease Multidisciplinary business.industry SARS-CoV-2 Health sciences Outbreak COVID-19 Isothermal titration calorimetry Virology Pharmaceutical science 030104 developmental biology Infectious disease (medical specialty) lcsh:H1-99 business 030217 neurology & neurosurgery Research Article lcsh:Q1-390 |
| Zdroj: | Heliyon, Vol 6, Iss 11, Pp e05421-(2020) Heliyon |
| ISSN: | 2405-8440 |
| Popis: | Corona virus disease 2019 (COVID-19) is a respiratory disease caused by a new coronavirus (SARS-CoV-2) which causes significant morbidity and mortality. The emergence of this novel and highly pathogenic SARS-CoV-2 and its rapid international spread poses a serious global public health emergency. To date 32,174,627 cases, of which 962,613 (2.99%) have died, have been reported (https://www.who.int/westernpacific/health-topics/coronavirus, accessed 23 Sep 2020). The outbreak was declared a Public Health Emergency of International Concern on 30 January 2020. There are still not many SARS-CoV-2-specific and effective treatments or vaccines available. A second round of infection is obviously unavoidable. Aptamers had already been at the centre of interest in the fight against viruses before now. The selection and development of a new aptamer is, however, a time-consuming process. We therefore checked whether a clinically developed aptamer, BC 007, which is currently in phase 2 of clinical testing for a different indication, would also be able to efficiently bind DNA-susceptible peptide structures from SARS-CoV-2-spreading crucial proteins, such as the receptor binding domain (RBD) of the spike protein and the RNA dependent RNA polymerase of SARS-CoV-2 (re-purposing). Indeed, several such sequence-sections have been identified. In particular for two of these sequences, BC 007 showed specific binding in a therapy-relevant concentration range, as shown in Nuclear magnetic resonance (NMR)- and Circular dicroism (CD)-spectroscopy and isothermal titration calorimetry (ITC). The excellent clinical toxicity and tolerability profile of this substance opens up an opportunity for rapid clinical testing of its COVID-19 effectiveness. Antiviral; Aptamer; BC 007; COVID-19; Re-purposing; SARS-CoV-2; Pharmaceutical science; Health sciences; Infectious disease; Pharmacology; Clinical research. |
| Databáze: | OpenAIRE |
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