Hypothermic Machine Perfusion Preservation of the DCD Kidney: Machine Effects
| Autor: | Martin J. Mangino, Susanne L. Lindell, John Brassil, Heather Muir |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Machine perfusion
Kidney medicine.medical_specialty Article Subject business.industry medicine.medical_treatment 030232 urology & nephrology Pulsatile flow Ischemia lcsh:Surgery lcsh:RD1-811 030230 surgery medicine.disease Nephrectomy Surgery Transplantation 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Anesthesia Vascular resistance Medicine business Perfusion Research Article |
| Zdroj: | Journal of Transplantation, Vol 2013 (2013) Journal of Transplantation |
| ISSN: | 2090-0007 |
| DOI: | 10.1155/2013/802618 |
| Popis: | Purpose. Kidneys from DCD donors represent a significant pool, but preservation problems exist. The study objective was to test the importance of machine type for hypothermic preservation of DCD kidneys.Methods. Adult Beagle dog kidneys underwent 45 minutes of warm in situ ischemia followed by hypothermic perfusion for 24 hours (Belzer-MPS Solution) on either an ORS LifePort or a Waters RM3 using standard perfusion protocols. Kidneys were then autotransplanted, and renal function was assessed over 7 days following contralateral nephrectomy.Results. Renal vascular resistance was not different between the two pumps. After 24 hours, the oxygen partial pressure and oxygen delivery in the LifePort perfusate were significantly lower than those in the RM3 but not low enough to change lactate production. TheLifePort ran significantly colder than RM3 (2° versus 5°C). The arterial pressure waveform of the RM3 was qualitatively different from the waveform of the LifePort. Preservation injury after transplantation was not different between the devices. When the LifePort was changed to nonpulsatile flow, kidneys displayed significantly greater preservation injury compared to RM3.Conclusions. Both LifePort and RM3 can be used for hypothermic machine perfusion preservation of DCD kidneys with equal outcomes as long as the duty cycle remains pulsatile. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|