Evolution of Antp-class genes and differential expression of Hydra Hox/paraHox genes in anterior patterning
| Autor: | Sylvia Kreger, Dominique Gauchat, Cédric Berney, Jan Pawlowski, Michèl Schummer, Brigitte Galliot, Francoise Mazet |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Molecular *Genes
Hydra Molecular Sequence Data Molecular Conserved Sequence Electrophoresis Transcription Factors/genetics ParaHox Biology Evolution Molecular Pulsed-Field ddc:590 Molecular Sequence Data *Multigene Family Homologous chromosome Animals Homeobox Amino Acid Sequence Cloning Molecular Hox gene Gene Homeodomain Proteins/*genetics Hydra/anatomy & histology/*classification/*genetics Conserved Sequence Phylogeny Body Patterning Genetics Homeodomain Proteins Gel Multidisciplinary Polarity (international relations) Phylogenetic tree Regeneration (biology) Genes Homeobox Nuclear Proteins Biological Sciences Body Patterning/*genetics Electrophoresis Gel Pulsed-Field Evolution Multigene Family Antennapedia Homeodomain Protein Lernaean Hydra Transcription Factors Cloning |
| Zdroj: | Proceedings of the National Academy of Sciences, Vol. 97, No 9 (2000) pp. 4493-4498 |
| ISSN: | 0027-8424 |
| Popis: | The conservation of developmental functions exerted by Antp-class homeoproteins in protostomes and deuterostomes suggested that homologs with related functions are present in diploblastic animals. Our phylogenetic analyses showed that Antp-class homeodomains belong either to non-Hox or to Hox/paraHox families. Among the 13 non-Hox families, 9 have diploblastic homologs, Msx , Emx , Barx , Evx , Tlx , NK-2 , and Prh/Hex , Not , and Dlx , reported here. Among the Hox/paraHox, poriferan sequences were not found, and the cnidarian sequences formed at least five distinct cnox families. Two are significantly related to the paraHox Gsx (cnox-2) and the mox (cnox-5) sequences, whereas three display some relatedness to the Hox paralog groups 1 (cnox-1), 9/10 (cnox-3) and the paraHox cdx (cnox-4). Intermediate Hox/paraHox genes (PG 3 to 8 and lox ) did not have clear cnidarian counterparts. In Hydra , cnox-1 , cnox-2 , and cnox-3 were not found chromosomally linked within a 150-kb range and displayed specific expression patterns in the adult head. During regeneration, cnox-1 was expressed as an early gene whatever the polarity, whereas cnox-2 was up-regulated later during head but not foot regeneration. Finally, cnox-3 expression was reestablished in the adult head once it was fully formed. These results suggest that the Hydra genes related to anterior Hox/paraHox genes are involved at different stages of apical differentiation. However, the positional information defining the oral/aboral axis in Hydra cannot be correlated strictly to that characterizing the anterior–posterior axis in vertebrates or arthropods. |
| Databáze: | OpenAIRE |
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