Whole-Genome Sequencing and Comparative Genomics of Three Helicobacter pylori Strains Isolated from the Stomach of a Patient with Adenocarcinoma
| Autor: | Palau de Miguel, Montserrat, Piqué i Clusella, Núria, Comeau, André M., Ramírez Lázaro, María José, Lario García, Sergio, Calvet, Xavier, Miñana i Galbis, David |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
virulence factors Virulence genomic comparison lcsh:Medicine Genome Article Aparell digestiu Bacterial genetics Genotype Immunology and Allergy CagA Gastrointestinal system diseases Molecular Biology Gene Comparative genomics Genetics Whole genome sequencing Genètica bacteriana Helicobacter pylori General Immunology and Microbiology biology lcsh:R biology.organism_classification Malalties del tracte gastrointestinal Digestive organs Infectious Diseases gastric adenocarcinoma |
| Zdroj: | Pathogens, Vol 10, Iss 331, p 331 (2021) Dipòsit Digital de la UB Universidad de Barcelona Pathogens Volume 10 Issue 3 |
| ISSN: | 2076-0817 |
| Popis: | Helicobacter pylori is a common pathogen associated with several severe digestive diseases. Although multiple virulence factors have been described, it is still unclear the role of virulence factors on H. pylori pathogenesis and disease progression. Whole genome sequencing could help to find genetic markers of virulence strains. In this work, we analyzed three complete genomes from isolates obtained at the same point in time from a stomach of a patient with adenocarcinoma, using multiple available bioinformatics tools. The genome analysis of the strains B508A-S1, B508A-T2A and B508A-T4 revealed that they were cagA, babA and sabB/hopO negative. The differences among the three genomes were mainly related to outer membrane proteins, methylases, restriction modification systems and flagellar biosynthesis proteins. The strain B508A-T2A was the only one presenting the genotype vacA s1, and had the most distinct genome as it exhibited fewer shared genes, higher number of unique genes, and more polymorphisms were found in this genome. With all the accumulated information, no significant differences were found among the isolates regarding virulence and origin of the isolates. Nevertheless, some B508A-T2A genome characteristics could be linked to the pathogenicity of H. pylori. Keywords: Helicobacter pylori; genomic comparison; virulence factors; gastric adenocarcinoma |
| Databáze: | OpenAIRE |
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