Esterases immobilized on aminosilane modified magnetic nanoparticles as a catalyst for biotransformation reactions
| Autor: | Deepthy Alex, Rajeev K. Sukumaran, Abraham Mathew |
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| Rok vydání: | 2014 |
| Předmět: |
Environmental Engineering
Aqueous solution Esterification Renewable Energy Sustainability and the Environment Ethyl acetate Esterases Bioengineering General Medicine Transesterification Silanes Enzymes Immobilized Catalysis Solvent chemistry.chemical_compound chemistry Biotransformation Biofuels Magnetic nanoparticles Organic chemistry Glutaraldehyde Magnetite Nanoparticles Waste Management and Disposal |
| Zdroj: | Bioresource technology. 167 |
| ISSN: | 1873-2976 |
| Popis: | Magnetite nanoparticles were prepared by reacting ferrous and ferric salts in presence of aqueous ammonia. The magnetic nanoparticles (MNPs) were amino functionalized by treating with 3-aminopropyl triethoxy silane (APTES) and was coupled with glutaraldehyde. A novel solvent tolerant esterase from Pseudozyma sp. NII 08165 was immobilized on the MNPs through covalent bonding to the glutaraldehyde. The magnetite nanoparticles had a size range of 10–100 nm, confirmed by DLS. Lipases immobilized on MNPs were evaluated for biotransformation reactions including synthesis of ethyl acetate and transesterification of vegetable oil for producing biodiesel. The MNP immobilized esterase had prolonged shelf life and there was no loss in enzyme activity. |
| Databáze: | OpenAIRE |
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