Dual lanthanide-doped complexes: the development of a time-resolved ratiometric fluorescent probe for anthrax biomarker and a paper-based visual sensor
| Autor: | Qi-Xian Wang, Shi-Fan Xue, Shengqiang Zhang, Zi-Han Chen, Guoyue Shi, Min Zhang, Shi-Hui Ma |
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| Rok vydání: | 2017 |
| Předmět: |
Paper
Lanthanide Biomedical Engineering Biophysics chemistry.chemical_element Nanotechnology Terbium Biosensing Techniques 02 engineering and technology 010402 general chemistry Lanthanoid Series Elements 01 natural sciences Anthrax chemistry.chemical_compound Europium Electrochemistry Humans Picolinic Acids Fluorescent Dyes Filter paper Chemistry business.industry Doping General Medicine Silicon Dioxide 021001 nanoscience & nanotechnology Dipicolinic acid Fluorescence 0104 chemical sciences Autofluorescence Optoelectronics Smartphone 0210 nano-technology business Biomarkers Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 94:388-393 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2017.03.027 |
| Popis: | In this work, a novel time-resolved ratiometric fluorescent probe based on dual lanthanide (Tb: terbium, and Eu: europium)-doped complexes (Tb/DPA@SiO2-Eu/GMP) has been designed for detecting anthrax biomarker (dipicolinic acid, DPA), a unique and major component of anthrax spores. In such complexes-based probe, Tb/DPA@SiO2 can serve as a stable reference signal with green fluorescence and Eu/GMP act as a sensitive response signal with red fluorescence for ratiometric fluorescent sensing DPA. Additionally, the probe exhibits long fluorescence lifetime, which can significantly reduce the autofluorescence interferences from biological samples by using time-resolved fluorescence measurement. More significantly, a paper-based visual sensor for DPA has been devised by using filter paper embedded with Tb/DPA@SiO2-Eu/GMP, and we have proved its utility for fluorescent detection of DPA, in which only a handheld UV lamp is used. In the presence of DPA, the paper-based visual sensor, illuminated by a handheld UV lamp, would result in an obvious fluorescence color change from green to red, which can be easily observed with naked eyes. The paper-based visual sensor is stable, portable, disposable, cost-effective and easy-to-use. The feasibility of using a smartphone with easy-to-access color-scanning APP as the detection platform for quantitative scanometric assays has been also demonstrated by coupled with our proposed paper-based visual sensor. This work unveils an effective method for accurate, sensitive and selective monitoring anthrax biomarker with backgroud-free and self-calibrating properties. |
| Databáze: | OpenAIRE |
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