Polycyclic aromatic hydrocarbons degradation by marine-derived basidiomycetes: optimization of the degradation process
| Autor: | Mariana Juventina Magrini, Gabriela A. L. Vieira, Lara Durães Sette, Rafaella Costa Bonugli-Santos, Marili V.N. Rodrigues |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Estadual de Campinas (UNICAMP), Instituto Latino Americano de Ciências da Vida e da Natureza |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Metabolite lcsh:QR1-502 010501 environmental sciences 01 natural sciences Microbiology lcsh:Microbiology Fungal Proteins 03 medical and health sciences chemistry.chemical_compound Bioremediation PAHs Cytochrome P-450 Enzyme System Marasmiellus Marine biotechnology Benzo(a)pyrene Seawater Polycyclic Aromatic Hydrocarbons Microbial biodegradation Phylogeny 0105 earth and related environmental sciences Pollutant Pyrenes Acute toxicity biology Chemistry Basidiomycota Fungi biology.organism_classification Experimental design Biodegradation Environmental 030104 developmental biology Environmental chemistry Epoxide Hydrolases Degradation (geology) Pyrene Research Paper |
| Zdroj: | Brazilian Journal of Microbiology, Volume: 49, Issue: 4, Pages: 749-756, Published: DEC 2018 Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Brazilian Journal of Microbiology Brazilian Journal of Microbiology v.49 n.4 2018 Sociedade Brasileira de Microbiologia (SBM) instacron:SBM Brazilian Journal of Microbiology, Vol 49, Iss 4, Pp 749-756 |
| ISSN: | 1517-8382 |
| Popis: | Made available in DSpace on 2018-12-11T16:53:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-01. Added 1 bitstream(s) on 2019-10-09T18:29:44Z : No. of bitstreams: 1 S1517-83822018000400749.pdf: 1052956 bytes, checksum: 34125206828746083f0a47a1e02819d0 (MD5) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Pyrene and benzo[a]pyrene (BaP) are high molecular weight polycyclic aromatic hydrocarbons (PAHs) recalcitrant to microbial attack. Although studies related to the microbial degradation of PAHs have been carried out in the last decades, little is known about degradation of these environmental pollutants by fungi from marine origin. Therefore, this study aimed to select one PAHs degrader among three marine-derived basidiomycete fungi and to study its pyrene detoxification/degradation. Marasmiellus sp. CBMAI 1062 showed higher levels of pyrene and BaP degradation and was subjected to studies related to pyrene degradation optimization using experimental design, acute toxicity, organic carbon removal (TOC), and metabolite evaluation. The experimental design resulted in an efficient pyrene degradation, reducing the experiment time while the PAH concentration applied in the assays was increased. The selected fungus was able to degrade almost 100% of pyrene (0.08 mg mL−1) after 48 h of incubation under saline condition, without generating toxic compounds and with a TOC reduction of 17%. Intermediate metabolites of pyrene degradation were identified, suggesting that the fungus degraded the compound via the cytochrome P450 system and epoxide hydrolases. These results highlight the relevance of marine-derived fungi in the field of PAH bioremediation, adding value to the blue biotechnology. Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) Instituto de Biociências Departamento de Bioquímica e Microbiologia Universidade Estadual de Campinas (UNICAMP) Centro Pluridisciplinar de Pesquisas Químicas Biológicas e Agrícolas Universidade Federal da Integração Latino-Americana (UNILA) Instituto Latino Americano de Ciências da Vida e da Natureza Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) Instituto de Biociências Departamento de Bioquímica e Microbiologia CAPES: 2010/50190-2 FAPESP: 2012/12622-3 CAPES: 2013/19486-0 CNPq: 2016/07957-7 |
| Databáze: | OpenAIRE |
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