The Enteropathogenic E. coli Effector EspB Facilitates Microvillus Effacing and Antiphagocytosis by Inhibiting Myosin Function
| Autor: | Yosuke Iizumi, Michinaga Ogawa, Chihiro Sasakawa, Hiroshi Sagara, Peter G. Gillespie, Yasuaki Kabe, Hiroshi Handa, Kanako Kume, Motoki Azuma, Takeshi Nagai |
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| Rok vydání: | 2007 |
| Předmět: |
Cancer Research
MICROBIO Molecular Sequence Data macromolecular substances Biology Myosins Microbiology digestive system Enteropathogenic Escherichia coli Mice Phagocytosis Virology Immunology and Microbiology(all) Myosin medicine Animals Humans Secretion Amino Acid Sequence Intestinal Mucosa Molecular Biology Actin Escherichia coli Infections Mice Inbred C3H Microvilli Virulence Effector Escherichia coli Proteins biochemical phenomena metabolism and nutrition Microvillus Actins medicine.anatomical_structure Cytoplasm Host cell cytoplasm Citrobacter rodentium Parasitology CELLBIO Female Caco-2 Cells Sequence Alignment Bacterial Outer Membrane Proteins HeLa Cells Protein Binding |
| Zdroj: | Cell Host & Microbe. 2(6):383-392 |
| ISSN: | 1931-3128 |
| DOI: | 10.1016/j.chom.2007.09.012 |
| Popis: | SummaryEnteropathogenic Escherichia coli (EPEC) destroys intestinal microvilli and suppresses phagocytosis by injecting effectors into infected cells through a type III secretion system (TTSS). EspB, a component of the TTSS, is also injected into the cytoplasm of host cells. However, the physiological functions of EspB within the host cell cytoplasm remain unclear. We show that EspB binds to myosins, which are a superfamily of proteins that interact with actin filaments and mediate essential cellular processes, including microvillus formation and phagocytosis. EspB inhibits the interaction of myosins with actin, and an EspB mutant that lacks the myosin-binding region maintained its TTSS function but could not induce microvillus effacing or suppress phagocytosis. Moreover, the myosin-binding region of EspB is essential for Citrobacter rodentium, an EPEC-related murine pathogen, to efficiently infect mice. These results suggest that EspB inhibits myosin functions and thereby facilitates efficient infection by EPEC. |
| Databáze: | OpenAIRE |
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