Immunophenotype of Abnormal Metaphases Demonstrating Multilineage Involvement in Myelodysplastic Syndromes
| Autor: | Dieter K. Hossfeld, H. J. Weh, Stefan Suciu, W. Zeller, Walter Fiedler |
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| Rok vydání: | 2016 |
| Předmět: |
Cancer Research
education.field_of_study Pathology medicine.medical_specialty medicine.drug_class Myelodysplastic syndromes Cell Population Chromosome Karyotype Hematology Biology Monoclonal antibody medicine.disease Staining medicine.anatomical_structure Immunophenotyping Oncology medicine education |
| Zdroj: | Leukemialymphoma. 2(3-4) |
| ISSN: | 1042-8194 |
| Popis: | Eight patients with myelodysplastic syndrome (MDS) and chromosome abnormalities were studied by a combined method which allows simultaneous analysis of the karyotype and immunophenotype of the same mitotic cell. To determine the cell lineages with abnormal karyotype, monoclonal antibodies in the alkaline phosphatase-antialkaline phosphatase (APAAP) staining methods were used All patients with MDS showed metaphases with abnormal karyotype in cells belonging to granulocytic/monocytic lineage. In 7 patients studied the leukaemic process also involved erythrocytic and/or megakaryocytic lineages. In 4 patients with 5q- chromosome it was evident that at least the granulocytic/monocytic and erythrocytic cell lineages participated in the clonal proliferation. One patient with 5q- chromosome at initial diagnosis showed a shift from megakaryocytic to a granulocytic/monocytic cell population at the time of acute leukaemia These findings indicate multi-lineage involvement in patients with MDS who have karyo-typic abnormalities. |
| Databáze: | OpenAIRE |
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