Water-soluble Manganese and Iron Mesotetrakis(carboxyl)porphyrin: DNA Binding, Oxidative Cleavage, and Cytotoxic Activities
| Autor: | Hai-Yang Liu, Jian-Ping Yang, Lei Shi, Wei Yin, Man-Li Cao, Jiang Tao, Yi-Yu Jiang, Yu-Qi Fang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Metalloporphyrins
Stereochemistry Iron Pharmaceutical Science chemistry.chemical_element porphyrin manganese iron DNA cytotoxicity Apoptosis Oxidative phosphorylation Manganese 010402 general chemistry 01 natural sciences Article Analytical Chemistry Metal chemistry.chemical_compound Cell Line Tumor Drug Discovery Humans Organic chemistry Physical and Theoretical Chemistry Hydrogen peroxide 010405 organic chemistry Chemistry Spectrum Analysis Organic Chemistry Water Porphyrin 0104 chemical sciences DNA-Binding Proteins Hep G2 Oxidative Stress Solubility Chemistry (miscellaneous) visual_art Proteolysis visual_art.visual_art_medium Molecular Medicine Hydroxyl radical Reactive Oxygen Species Oxidation-Reduction Protein Binding |
| Zdroj: | Molecules; Volume 22; Issue 7; Pages: 1084 Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules22071084 |
| Popis: | Two new water-soluble metal carboxyl porphyrins, manganese (III) meso-tetrakis (carboxyl) porphyrin and iron (III) meso-tetrakis (carboxyl) porphyrin, were synthesized and characterized. Their interactions with ct-DNA were investigated by UV-Vis titration, fluorescence spectra, viscosity measurement and CD spectra. The results showed they can strongly bind to ct-DNA via outside binding mode. Electrophoresis experiments revealed that both complexes can cleave pBR322 DNA efficiently in the presence of hydrogen peroxide, albeit 2-Mn exhibited a little higher efficiency. The inhibitor tests suggest the oxidative DNA cleavage by these two complexes may involve hydroxyl radical active intermediates. Notably, 2-Mn exhibited considerable photocytotoxicity against Hep G2 cell via triggering a significant generation of ROS and causing disruption of MMP after irradiation. |
| Databáze: | OpenAIRE |
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