Sensitive Analysis of Sialic Acid and Related Compound by Hydrophilic Interaction Liquid Chromatography Using Fluorescence Detection after Derivatization with DBD-PZ
| Autor: | Kazuya Nakagomi, Motonobu Satoh, Makoto Yasuda, Akane Kawasaki, Kiyoko Kaneko, Ryosuke Iijima, Ken-ichi Mawatari, Noriko Yamaoka, Tomoko Fukuuchi, Satoru Yui |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Sensitive analysis High-performance liquid chromatography Fluorescence Piperazines Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Hydrogen peroxide Derivatization Oxadiazoles Sulfonamides Chromatography Molecular Structure Chemistry Hydrophilic interaction chromatography eye diseases Sialic acid 030104 developmental biology 030228 respiratory system Sialic Acids N-Acetylneuraminic acid Hydrophobic and Hydrophilic Interactions Chromatography Liquid |
| Zdroj: | Analytical sciences : the international journal of the Japan Society for Analytical Chemistry. 34(7) |
| ISSN: | 1348-2246 |
| Popis: | N-Acetylneuraminic acid (NANA) has been reported to react with hydrogen peroxide in vitro to produce 4-(acetylamino)-2,4-dideoxy-D-glycero-D-galacto-octonic acid (ADOA). We labeled NANA and ADOA with 4-(N,N-dimethylaminosulfonyl)-7-piperazino-2,1,3-benzoxadiazole (DBD-PZ) for simultaneous detection. The derivatized NANA and ADOA were separated using hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection. The calibration curves of DBD-PZ-derivatized NANA and ADOA showed good linearity in the range of 221 fmol to 1.5 nmol, and 44 fmol to 1.5 nmol, respectively. This analytical method has high specificity and is useful for the detection of NANA and ADOA in saliva and serum. |
| Databáze: | OpenAIRE |
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