LncRNA UCA1 inhibits epilepsy and seizure-induced brain injury by regulating miR-495/Nrf2-ARE signal pathway
| Autor: | Jun-jie Geng, Hai-biao Zhao, Xian-zhi Liu, Jie-feng Geng, Wen-fei Fan, Xing Liu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
NF-E2-Related Factor 2 Endogeny Apoptosis Hippocampal formation Muscarinic Agonists Biochemistry Hippocampus Flow cytometry Rats Sprague-Dawley 03 medical and health sciences Epilepsy 0302 clinical medicine Western blot Seizures medicine Animals Cells Cultured Neurons medicine.diagnostic_test Chemistry Pilocarpine Cell Biology Transfection medicine.disease Antioxidant Response Elements Cell biology Rats MicroRNAs 030104 developmental biology Gene Expression Regulation Brain Injuries RNA Long Noncoding 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | The international journal of biochemistrycell biology. 99 |
| ISSN: | 1878-5875 |
| Popis: | Background Both LncRNA UCA1 and miR-495 are crucial gene regulators in various disorders. This study aims to investigate their role in epilepsy and seizure-induced brain injury. Methods In this research, rat model of epilepsy was established by pilocarpine induction. The RNA and protein expression in hippocampal tissues and neurons were determined by qRT-PCR and western blot, respectively. The hippocampal neurons were isolated from hippocampal tissues, and treated with magnesium-free (MGF) physiological solution for epileptiform activity induction. The endogenous expression of related genes was modulated by recombinant plasmids and cell transfection. Flow cytometry was used to analyze the cell apoptosis. Dual luciferase reporter assay was performed to determine the interaction between miR-495 and Nrf2 in HEK-293 cells. Results The lncRNA UCA1 and Nrf2 were down-regulated in epileptiform hippocampal tissues and neurons, while the miR-495 was up-regulated. Over-expression of UCA1 inhibited apoptosis of hippocampal neurons by suppressing miR-495. MiR-495 negatively regulated Nrf2. UCA1 inhibited apoptosis of hippocampal neurons through miR-495/Nrf2-ARE pathway. UCA1 suppressed pilocarpine-induced epilepsy in rat. Conclusion LncRNA UCA1 suppressed pilocarpine-induced epilepsy by inhibiting apoptosis of hippocampal neurons through miR-495/Nrf2-ARE pathway, and thereby inhibiting brain injury induced by seizure. |
| Databáze: | OpenAIRE |
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