Tracing of a neuronal network in the locust by pressure injection of markers into a synaptic neuropil
Autor: | Ralf Heinrich, Kirsten Jacobs, Reinhard Lakes-Harlan |
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Rok vydání: | 1998 |
Předmět: |
Nervous system
Auditory Pathways Neuropil Microinjections Grasshoppers Biology Synapse 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Postsynaptic potential Biocytin medicine Biological neural network Animals Neurons Afferent 030304 developmental biology 0303 health sciences General Neuroscience Thorax biology.organism_classification Ganglia Invertebrate medicine.anatomical_structure nervous system chemistry Acoustic Stimulation Synapses Neuron Nerve Net Neuroscience Microelectrodes 030217 neurology & neurosurgery Locust Biomarkers |
Zdroj: | Journal of neuroscience methods. 80(1) |
ISSN: | 0165-0270 |
Popis: | Central neuronal circuits of vertebrates have often been investigated using injection of markers into synaptic neuropils, whereas similar techniques have rarely been applied in invertebrates. In this study we tested several neuroanatomical tracers for their ability to mark central neuronal circuits in insects, using the well described auditory network of the locust, Locusta migratoria. After physiological localization of an auditory neuropil various tracers were pressure injected. Horseradish peroxidase, dextrans (3 and 10 kDa) and especially biocytin and neurobiotin were effectively incorporated by auditory interneurons, which resulted in their extensive labeling. Postsynaptic regions turned out to be the major, if not exclusive sites of uptake of injected markers, which is deduced from two lines of evidence: (i) for labeling of identified auditory neurons it was necessary to apply the tracer to postsynaptic sites of the neuron; (ii) only a few non-auditory neurons were labeled (probably by lesioning axons during electrode impalement). No evidence could be found for an activity dependent uptake. We conclude that pressure injection of certain tracers into synaptic areas can be used to identify central nervous circuits in insects. |
Databáze: | OpenAIRE |
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