New Insights into the Methodology of L-Arginine-Induced Acute Pancreatitis
| Autor: | Tamás Takács, Béla Vasas, Eszter T. Vegh, Viktória Venglovecz, Béla Iványi, Péter Hegyi, Eszter S. Kormányos, Zoltán Rakonczay, Petra Pallagi, Balázs Kui, Zsolt Balla |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_specialty Necrosis Arginine lcsh:Medicine Inflammation Mice Disease severity Internal medicine medicine Animals 03.02. Klinikai orvostan lcsh:Science Pancreas Peroxidase Mice Inbred BALB C Multidisciplinary Pancreatitis Acute Necrotizing business.industry lcsh:R Histology medicine.disease Mice Inbred C57BL Disease Models Animal Endocrinology medicine.anatomical_structure Amylases Immunology Acute pancreatitis Pancreatitis Female lcsh:Q medicine.symptom business Research Article |
| Zdroj: | PLoS ONE, Vol 10, Iss 2, p e0117588 (2015) PLoS ONE |
| Popis: | Animal models are ideal to study the pathomechanism and therapy of acute pancreatitis (AP). The use of L-arginine-induced AP model is nowadays becoming increasingly popular in mice. However, carefully looking through the literature, marked differences in disease severity could be observed. In fact, while setting up the L-arginine (2×4 g/kg i.p.)-induced AP model in BALB/c mice, we found a relatively low rate (around 15%) of pancreatic necrosis, whereas others have detected much higher rates (up to 55%). We suspected that this may be due to differences between mouse strains. We administered various concentrations (5–30%, pH = 7.4) and doses (2×4, 3×3, or 4×2.5 g/kg) of L-arginine-HCl in BALB/c, FVB/n and C57BL/6 mice. The potential gender-specific effect of L-arginine was investigated in C57BL/6 mice. The fate of mice in response to the i.p. injections of L arginine followed one of three courses. Some mice (1) developed severe AP or (2) remained AP-free by 72 h, whereas others (3) had to be euthanized (to avoid their death, which was caused by the high dose of L-arginine and not AP) within 12 h., In FVB/n and C57BL/6 mice, the pancreatic necrosis rate (about 50%) was significantly higher than that observed in BALB/c mice using 2×4 g/kg 10% L–arginine, but euthanasia was necessary in a large proportion of animals, The i.p. injection of lower L-arginine concentrations (e.g. 5–8%) in case of the 2×4 g/kg dose, or other L-arginine doses (3×3 or 4×2.5 g/kg, 10%) were better for inducing AP. We could not detect any significant differences between the AP severity of male and female mice. Taken together, when setting up the L-arginine-induced AP model, there are several important factors that are worth consideration such as the dose and concentration of the administered L arginine-HCl solution and also the strain of mice. |
| Databáze: | OpenAIRE |
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