The immunomodulatory activities of licorice polysaccharides (Glycyrrhiza uralensis Fisch.) in CT 26 tumor-bearing mice
| Autor: | Ying Zhao, Peter Mwitari Githaiga, Yuhong Bian, Peter Amwoga Ayeka |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Chemokine Normal diet medicine.medical_treatment Population Antineoplastic Agents Thymus Gland Pharmacology Immunomodulation Mice Licorice 03 medical and health sciences 0302 clinical medicine Immune system Polysaccharides Cell Line Tumor medicine Animals Immunologic Factors Glycyrrhiza uralensis Interleukin 6 education Mice Inbred BALB C education.field_of_study Tumor biology Plant Extracts Chemistry Neoplasms Experimental lcsh:Other systems of medicine General Medicine lcsh:RZ201-999 biology.organism_classification Anticancer 030104 developmental biology Cytokine Complementary and alternative medicine 030220 oncology & carcinogenesis biology.protein Cytokines Tumor necrosis factor alpha Spleen Research Article |
| Zdroj: | BMC Complementary and Alternative Medicine, Vol 17, Iss 1, Pp 1-9 (2017) BMC Complementary and Alternative Medicine |
| ISSN: | 1472-6882 |
| DOI: | 10.1186/s12906-017-2030-7 |
| Popis: | Background The increasing use of complementary and alternative medicine (CAM) has kindled the need for scientific evaluation of the mechanism of action of CAMs. Although, licorice, a common ingredient in many Traditional Chinese medicine (TCM) has attracted great attention for its antitumor and immunomodulatory activities, the mechanism of action of its polysaccharides is still unclear. Here we report the immunomodulatory activity of licorice polysaccharides in vivo. Methods The differential anticancer activities of licorice polysaccharides by tumorigenesis and immunomodulation was evaluated in vivo. Six weeks old, 120 CT-26 tumor bearing BALB/c mice, weighing 20 ± 2 g were used. They were randomly divided into six groups, three groups receiving high molecular weight (fraction A), low molecular weight (fraction B) polysaccharides and crude extract (fraction C); positive, negative and normal groups receiving cytoxin, saline and normal diet respectively. Weight of mice and tumors was determined and tumorigenicity assay calculated to determine the anticancer effects. Immunomodulatory potential was determined by immune organ indices, immune cell population and serum cytokine levels using immune organ weight and index, flow cytometry and cytokine/chemokine bead panel kit respectively. Results Licorice polysaccharides exhibited immunomodulatory activities in CT 26 tumor bearing BALB/c mice. The polysaccharides significantly suppressed tumor growth and increased immune organ index. Furthermore, the immunomodulatory effect was evident with activation of CD4+ and CD8+ immune cells population. The polysaccharides also affected the production of various cytokines, by increasing IL 2, IL 6, IL 7 levels and a decreasing TNFα levels. Conclusion In summary, licorice polysaccharide especially of low molecular weight exhibit anticancer and immunomodulatory activities by suppressing tumor growth and improving general health of mice. They also augment the thymus/spleen index and population of T lymphocytes. Furthermore, the polysaccharides enhance the levels of serum antitumor cytokines, IL 2, IL 6 and IL 7 while decreasing pro-tumor cytokine TNFα. |
| Databáze: | OpenAIRE |
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