Draft genome assembly of the Aral barbell Luciobarbus brachycephalus using PacBio sequencing
| Autor: | Minghui Meng, Ming Zou, Wei Xu, Longwu Geng, Haifeng Jiang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
AcademicSubjects/SCI01140
genome annotation Cyprinidae Sequence assembly Biology de novo assembly phylogeny Genome Genome Size Phylogenetics Genetics Luciobarbus Animals Genome size Gene Ecology Evolution Behavior and Systematics PacBio sequencing Contig AcademicSubjects/SCI01130 Molecular Sequence Annotation Genome project Sequence Analysis DNA biology.organism_classification Genome Report Evolutionary biology Luciobarbus brachycephalus |
| Zdroj: | Genome Biology and Evolution |
| ISSN: | 1759-6653 |
| Popis: | The endangered Aral barbell Luciobarbus brachycephalus is endemic to the water systems of the Caspian Sea and Aral Sea. Given the scarcity of genetic data for the species, we present a draft assembly based on PacBio long read sequencing technology. Approximate 299.4 Gb of long reads representing 166X of the estimated genome size were generated, and the final assembly was composed of 653 contigs totaling approximately 1,698.3 Mb, with a contig N50 length of 4.5 Mb. A total of 807.6 Mb represented approximately 47.6% of the assembly and were identified as repeats. Fifty-four thousand and six hundred possible protein genes were predicted, among which 50,727, representing approximately 92.9%, could be annotated by at least one database. Evolutionary analysis showed that L. brachycephalus and Labeo rohita diverged by approximately 42.6 Mya, and the obvious expansion of gene families residing in the L. brachycephalus genome may be attributed to the specific whole genome duplication of the species. The first genome assembly of L. brachycephalus can not only provide a foundation for genetic conservation and molecular breeding of this species but also contribute to comparative analyses of genome biology and evolution within Cyprinidae. |
| Databáze: | OpenAIRE |
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