Determination of diltiazem hydrochloride in human serum by high-performance liquid chromatography
| Autor: | S.S. Gangwal, Y.N. Shah, R.S. Chaudhary, K.C. Jindal, M.K. Avachat |
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| Rok vydání: | 1993 |
| Předmět: |
Chromatography
Acetonitriles Chemistry Elution General Chemistry Reversed-phase chromatography High-performance liquid chromatography chemistry.chemical_compound Diltiazem Kinetics Pharmacokinetics Ethanolamines Triethanolamine medicine Humans Sodium Hydroxide Diltiazem hydrochloride Acetonitrile Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of chromatography. 614(2) |
| Popis: | A simple and sensitive reversed-phase high-performance liquid chromatographic method for the determination of diltiazem in human serum has been developed. The method involves a one-step deproteinization of serum for sample clean-up using acetonitrile. A LiChrosorb RP-8 column (30 cm × 4.1 mm I.D.) was eluted isocratically with acetonitrile—0.01 M dibasic sodium phosphate (40:60, v/v) containing 0.01% triethanolamine. Diltiazem was monitored at 237 nm and 0.1 a.u.f.s. The completion time for assay was less than 15 min, and the lower limit of quantitation was 10 ng/ml for a 100-μl injection volume. Using this method, the pharmacokinetic parameters were calculated from a serum concentration versus time profile of diltiazem. |
| Databáze: | OpenAIRE |
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