Establishment of the B cell precursor acute lymphoblastic leukemia cell line MUTZ-5 carrying a (12;13) translocation

Autor: Martin J. S. Dyer, Hans G. Drexler, Hilmar Quentmeier, Roderick A.F. MacLeod, Corinna Meyer, L. J. Coignet, J. W. G. Janssen
Rok vydání: 2001
Předmět:
Zdroj: Leukemia. 15:1471-1474
ISSN: 1476-5551
0887-6924
DOI: 10.1038/sj.leu.2402212
Popis: Continuous leukemia-lymphoma cell lines are important research tools, in particular as starting material for the cloning of recurrent translocations. In 1998, we established the continuous leukemia cell line MUTZ-5. The two cell lines MUTZ-6 and MUTZ-7 were derived from the same primary specimen and are hence simultaneous sister cell lines. The primary specimen was obtained from the peripheral blood of a 26-year-old man with B-cell precursor-acute lymphoblastic leukemia at relapse carrying a t(12;13). The immu- noprofile of MUTZ-5 cells as determined by flow cytometry was as follows; B-cell markers: CD10+, CD19+, CD20+, CD22+, CD37+, CD40+, cyCD79a+, cyCD79b+, CD138+, cyCD179b+, surface/cylgM; except for CD33, negative for T-/NK cell, myelomonocytic, erythroid and megakaryocytic markers; progenitor-activation markers: CD30+, CD34((+)), CD38+, CD71+, HLA-DR+, TdT+. This immun- oprofile corresponds to that of a precursor B- cell. Cytokine receptor expression: CD119+ (IFN-γR), CD127+ (IL-7R), CD135+ (FLT3). Despite receptor expression, IFN-γ, IL-7 or FLT3 ligand did not enhance proliferation, nor did so any other stimulatory cytokine. Several cytokines (IL-4, TGF-β31, TNF-α, TNF-β) had significant inhibitory effects. The immunoglobulin heavy chain gene was found to be rearranged. Giemsa-banding cytogenetics showed the following karyotype which was identical in all three sister cell lines: 45 X, -Y, t(12;13)(pl2;ql3-14). The karyotype and DNA fingerprinting confirmed the malignant nature and the authenticity of the cell line, excluding cross-contamination with other cells. MUTZ-5 represents a new unique leukemia B-cell line; its scientific significance lies in the t(12;13).
Databáze: OpenAIRE