Simultaneous Determination of Phenobarbital, Phenytoin, Carbamazepine and Carbamazepine-10,11-epoxide in Plasma of Epileptic Patients
| Autor: | Mohammad Barzegar, Shadi Shiva, Azam Samadi, Abolghasem Jouyban, Shahram Sadeghvand, Maryam Khoubnasabjafari |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Phenytoin
therapeutic drug monitoring Potassium lcsh:RS1-441 Pharmaceutical Science Epoxide chemistry.chemical_element phenobarbital carbamazepine metabolite 030226 pharmacology & pharmacy 01 natural sciences lcsh:Pharmacy and materia medica 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine General Pharmacology Toxicology and Pharmaceutics PHENOBARBITAL/PHENYTOIN Chromatography medicine.diagnostic_test hplc analysis 010401 analytical chemistry phenytoin Carbamazepine 0104 chemical sciences chemistry Therapeutic drug monitoring carbamazepine Phenobarbital Carbamazepine epoxide medicine.drug |
| Zdroj: | Pharmaceutical Sciences, Vol 25, Iss 4, Pp 345-351 (2019) |
| ISSN: | 2383-2886 1735-403X |
| Popis: | Background: Quantitative analyses of antiepileptic drugs are required in clinic and to rational dosage adjustment, the clinician needs the blood levels of these drugs. A high-performance liquid chromatography with spectrophotometric detection has been developed and validated for simultaneous determination of some antiepileptic drugs in plasma of patients with epilepsy. Methods: A simple procedure based on deproteinization by acetonitrile was used for pre-treatment of plasma samples. Liquid chromatographic analysis was carried out on a Nova-Pak® C18 analytical column, using a ternary mixture of potassium dihydrogen phosphate buffer (pH 6.0)-acetonitrile-2-propanol (63:22:15, v/v/v) as the mobile phase, at a flow rate of 1.0 mL min-1. Results: Calibration curves were linear over a range of 1–40 µg mL-1 for phenobarbital, 1–30 µg mL-1 for phenytoin, 0.3–15 µg mL-1 for carbamazepine and 0.5–6 µg mL-1 for carbamazepine epoxide. Conclusion: The simple sample pre-treatment, combined with the fast chromatographic run was used for the determination of antiepileptic drugs for a large number of patient samples. |
| Databáze: | OpenAIRE |
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