Esterase gene expression in Chinese hamster and mouse lymphoma hybrids isolated under nonselective pressure
| Autor: | S. R. Ayad, J. G. Delinassios |
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| Rok vydání: | 1974 |
| Předmět: |
Time Factors
Lymphoma Genetic Linkage Esterase Gene Population Mitosis Hamster Hybrid Cells Biochemistry Chromosomes Chinese hamster Cell Line Cell Fusion Polyploidy Mice Genetic linkage Cricetinae Culture Techniques Gene expression Methods Genetics Animals education Molecular Biology Gene Ecology Evolution Behavior and Systematics Cell Nucleus education.field_of_study biology Esterases General Medicine Fibroblasts Electrophoresis Disc biology.organism_classification Molecular biology Evaluation Studies as Topic Cell culture Karyotyping Cell Division |
| Zdroj: | Biochemical Genetics. 12:147-161 |
| ISSN: | 1573-4927 0006-2928 |
| Popis: | Hybrids between a fibroblastic Chinese hamster cell line (CH23) and a mouse lymphoma cell line (P388F36) were produced and isolated by a simple new method without using selective media and avoiding contact with the parental cells. The chromosomal situation in the two hybrid types (PCM and PCS) isolated suggested that growth on glass surface (PCM) or in suspension (PCS) depended on the number of hamster and mouse chromosomes which existed in the hybrids. Chromosomal stability in hybrids grown as monolayers (PCM) was reached at a stage in which two to four mouse chromosomes coexisted with no fewer than 19 hamster chromosomes. In a study of gene linkage utilizing clones of this hybrid population, five out of nine genes regulating the synthesis of different esterases in the mouse cells used were found to be unlinked. |
| Databáze: | OpenAIRE |
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