Bioconjugation with Maleimides: A Useful Tool for Chemical Biology
| Autor: | João M. J. M. Ravasco, Pedro M. P. Gois, Hélio Faustino, Alexandre F. Trindade |
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| Rok vydání: | 2018 |
| Předmět: |
Bioconjugation
Immunoconjugates 010405 organic chemistry Organic Chemistry Disulfide bond Chemical biology Rational design General Chemistry 010402 general chemistry 01 natural sciences Combinatorial chemistry Catalysis Antibodies 0104 chemical sciences Maleimides chemistry.chemical_compound chemistry Humans Sulfhydryl Compounds Somatostatin Maleimide Serum Albumin |
| Zdroj: | Chemistry (Weinheim an der Bergstrasse, Germany). 25(1) |
| ISSN: | 1521-3765 |
| Popis: | Maleimide chemistry stands out in the bioconjugation toolbox by virtue of its synthetic accessibility, excellent reactivity, and practicability. The second-generation of clinically approved antibody-drug conjugates (ADC) and much of the current ADC pipeline in clinical trials contain the maleimide linkage. However, thiosuccinimide linkages are now known to be less robust than once thought, and ergo, are correlated with suboptimal pharmacodynamics, pharmacokinetics, and safety profiles in some ADC constructs. Rational design of novel generations of maleimides and maleimide-type reagents have been reported to address the shortcomings of classical maleimides, allowing for the formation of robust bioconjugate linkages. This review highlights the main strategies for rational reagent design that have allowed irreversible bioconjugations in cysteines, reversible labelling strategies and disulfide re-bridging. |
| Databáze: | OpenAIRE |
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