The role of testosterone in the respiratory and thermal responses to hypoxia and hypercapnia in rats
| Autor: | Carolina da Silveira Scarpellini, Raphael E. Szawka, Luis Gustavo Alexandre Patrone, Kênia C. Bícego, Danuzia A. Marques, Luciane H. Gargaglioni |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), St Michaels Hosp, Universidade Federal de Minas Gerais (UFMG) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty breathing Endocrinology Diabetes and Metabolism 030209 endocrinology & metabolism Hypoxic ventilatory response Flutamide Hypercapnia 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Oxygen Consumption Internal medicine Hyperventilation medicine Androgen Receptor Antagonists Animals Testosterone Normocapnia Rats Wistar Hypoxia Carotid Body business.industry androgens Hypoxia (medical) Rats Androgen receptor chemosensitivity 030104 developmental biology chemistry Receptors Androgen Respiratory Physiological Phenomena gonadal hormone medicine.symptom business Orchiectomy Body Temperature Regulation |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2021-06-25T12:20:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Many diseases of the respiratory system occur differently in males and females, indicating a possible role of gonadal hormones in respiratory control. We hypothesized that testosterone (T) is important for the ventilatory chemosensitivity responses in males. To test this hypothesis, we evaluated ventilation ((E)), metabolic rate and body temperature (T-b) under normoxia/normocapnia, hypercapnia and hypoxia in orchiectomized (ORX), ORX with testosterone replacement (ORX + T) or flutamide (FL, androgen receptor blocker)-treated rats. We also performed immunohistochemistry to evaluate the presence of androgen receptor (AR) in the carotid body ( CB) of intact males. Orchiectomy promoted a reduction (E) and ventilatory equivalent ((E)/O-2) under room-air conditions, which was restored with testosterone treatment. Moreover, during hypoxia or hypercapnia, animals that received testosterone replacement had a higher (E) and (E)/O-2 than control and ORX, without changes in metabolic and thermal variables. Flutamide decreased the hypoxic ventilatory response without changing the CO2-drive to breathe, suggesting that the testosterone effect on hypercapnic hyperventilation does not appear to involve the AR. We also determined the presence of AR in the CB of intact animals. Our findings demonstrate that testosterone seems to be important for maintaining resting (E) in males. In addition, the influence of testosterone on (E), either during resting conditions or under hypoxia and hypercapnia, seems to be a direct and specific effect, as no changes in metabolic rate or T-b were observed during any treatment. Finally, a putative site of testosterone action during hypoxia is the CB, since we detected the presence of AR in this structure. Sao Paulo State Univ, Fac Agr & Veterinarian Sci, Dept Anim Morphol & Physiol, Jaboticabal, SP, Brazil St Michaels Hosp, Brain & Breathing Sci Lab, Keenan Res Ctr Biomed Sci, Toronto, ON, Canada Fed Univ Minas Gerais UFMG, Inst Biol Sci, Dept Physiol & Biophys, Belo Horizonte, MG, Brazil Sao Paulo State Univ, Fac Agr & Veterinarian Sci, Dept Anim Morphol & Physiol, Jaboticabal, SP, Brazil FAPESP: 2019/09469-8 CNPq: CNPq -407490/2018-3 FAPESP: 2016/04276-9 CNPq: 140715/2015-0 |
| Databáze: | OpenAIRE |
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