Loss of heterozygosity in acute leukemia: evidence of frequent submicroscopic deletions
| Autor: | Lucia Cascio, Rosaria Basiricò, Giuseppe Cammarata, Anna Marfia, Maria La Rosa, Alessandra Santoro, Valentina Rizzo, Cecilia Agueli, Francesco Fabbiano, Salvo Mirto |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Loss of Heterozygosity Biology Loss of heterozygosity chemistry.chemical_compound hemic and lymphatic diseases Internal medicine medicine Humans Child Allelotype neoplasms Aged Sequence Deletion Chromosome Aberrations Genetics Acute leukemia Hematology Myeloid leukemia Middle Aged Molecular biology Clone Cells chemistry Leukemia Myeloid Acute Disease Allelic Imbalance Microsatellite Female DNA Microsatellite Repeats |
| Zdroj: | Haematologica. 92:678-681 |
| ISSN: | 1592-8721 0390-6078 |
| Popis: | Although chromosomal abnormalities are detected by conventional cytogenetic analysis (CCA) in 40-60% of patients with acute myeloid leukemia (AML), cryptic chromosomal deletions may only be detected by molecular analysis. To determine their frequency, we studied 74 cases of AML by microsatellite allelotype assay using 35 microsatellites spanning eight chromosomal regions known to be frequently involved in AML. In 42 (57%) we found DNA imbalance at the screened loci. This was detected by CCA only in 4 cases. Our data show that cryptic deletions are a common event in AML. |
| Databáze: | OpenAIRE |
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