Box C/D small nucleolar RNA trafficking involves small nucleolar RNP proteins, nucleolar factors and a novel nuclear domain

Autor: Evelyne Bertrand, Marie Françoise Thiry, David Tollervey, Céline Verheggen, Jean-Marie Blanchard, John Mouaikel, Denis L. J. Lafontaine, Rémy Bordonné
Přispěvatelé: Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Laboratoire de Physique Statistique de l'ENS (LPS), Université Paris Diderot - Paris 7 (UPD7)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Fédération de recherche du Département de physique de l'Ecole Normale Supérieure - ENS Paris (FRDPENS), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Fédération de recherche du Département de physique de l'Ecole Normale Supérieure - ENS Paris (FRDPENS), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2001
Předmět:
Saccharomyces cerevisiae Proteins
Nucleolus
Small Nucleolar/*metabolism Saccharomyces cerevisiae *Saccharomyces cerevisiae Proteins
Active Transport
Cell Nucleus
RNA-binding protein
Coiled Bodies
Saccharomyces cerevisiae
Biology
Cell Nucleus Cell Compartmentation Cell Nucleolus/*metabolism Coiled Bodies/metabolism Fungal Proteins/metabolism Models
Models
Biological
General Biochemistry
Genetics and Molecular Biology
Article
Fungal Proteins
03 medical and health sciences
Ribonucleoproteins
Small Nucleolar
RNA
Small Nucleolar
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Small nucleolar RNA
Molecular Biology
030304 developmental biology
Fibrillarin
0303 health sciences
Fungal protein
Nucleoplasm
General Immunology and Microbiology
Serine-Arginine Splicing Factors
urogenital system
General Neuroscience
030302 biochemistry & molecular biology
Nuclear Proteins
RNA-Binding Proteins
Phosphoproteins
Molecular biology
Small Nucleolar/*metabolism *RNA-Binding Proteins Ribonucleoproteins
Cell biology
Cell Compartmentation
Cajal body
Biological Nuclear Proteins/metabolism Phosphoproteins/metabolism RNA
Nucleolin
Active Transport
Cell Nucleolus
Zdroj: EMBO Journal
EMBO Journal, EMBO Press, 2001, 20 (19), pp.5480--90. ⟨10.1093/emboj/20.19.5480⟩
EMBO Journal, 2001, 20 (19), pp.5480--90. ⟨10.1093/emboj/20.19.5480⟩
ISSN: 0261-4189
1460-2075
DOI: 10.1093/emboj/20.19.5480⟩
Popis: International audience; Nucleolar localization of box C/D small nucleolar (sno) RNAs requires the box C/D motif and, in vertebrates, involves transit through Cajal bodies (CB). We report that in yeast, overexpression of a box C/D reporter leads to a block in the localization pathway with snoRNA accumulation in a specific sub-nucleolar structure, the nucleolar body (NB). The human survival of motor neuron protein (SMN), a marker of gems/CB, specifically localizes to the NB when expressed in yeast, supporting similarities between these structures. Box C/D snoRNA accumulation in the NB was decreased by mutation of Srp40 and increased by mutation of Nsr1p, two related nucleolar proteins that are homologous to human Nopp140 and nucleolin, respectively. Box C/D snoRNAs also failed to accumulate in the NB, and became delocalized to the nucleoplasm, upon depletion of any of the core snoRNP proteins, Nop1p/fibrillarin, Snu13p, Nop56p and Nop5p/Nop58p. We conclude that snoRNP assembly occurs either in the nucleoplasm, or during transit of snoRNAs through the NB, followed by routing of the complete snoRNP to functional sites of ribosome synthesis.
Databáze: OpenAIRE
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