Thomsen-Friedenreich-related carbohydrate antigens in normal adult human tissues: a systematic and comparative study
| Autor: | Yi Cao, Georg F. Springer, Peter Stosiek, Uwe Karsten |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Adult
Isoantigens Histology medicine.drug_class Molecular Sequence Data Ribosome Inactivating Proteins Alpha (ethology) Cross Reactions Monoclonal antibody Epithelium ABO Blood-Group System Immunoenzyme Techniques Antigen Lectins medicine Humans Antigens Tumor-Associated Carbohydrate Molecular Biology Thomsen-Friedenreich Antigen biology Histocytochemistry Antibodies Monoclonal Lectin Epithelial Cells Cell Biology Molecular biology Medical Laboratory Technology Carbohydrate Sequence Organ Specificity Ribosome Inactivating Proteins Type 1 Jacalin biology.protein Immunohistochemistry Plant Lectins Antibody Trisaccharides |
| Zdroj: | Histochemistry and Cell Biology. 106:197-207 |
| ISSN: | 1432-119X 0948-6143 |
| DOI: | 10.1007/bf02484401 |
| Popis: | A broad variety of normal human tissues were examined for the expression of Thomsen-Friedenreich (TF)-related histo-blood group antigens, TF (Gal beta 1-3GalNAc alpha 1-R), Tn (TF precursor, GalNAc alpha 1-R), sialosyl-Tn (NeuAc alpha 2-6GalNAc alpha 1-R), considered to be useful in cancer diagnosis and immunotherapy, and sialosyl-TF, the cryptic form of TF. These antigens or, more correctly, glycotopes, were determined by immunohistochemistry with at least two monoclonal antibodies (mAbs) each (except sialosyl-TF) as well as by lectin histochemistry. For a better dissection of sialosyl-TF and TF glycotopes, tissue sections were pretreated with galactose oxidase or the galactose oxidase-Schiff sequence. Staining with mAbs appeared to be more restricted than with the lectins used. Distribution patterns among normal epithelia were different for all four antigens. These antigens were also detected in some non-epithelial tissues. They can be classified in the following sequence according to the frequency of their occurrence in normal tissues: sialosyl-TF > > sialosyl-Tn > Tn > TF. Most of the positively staining sites for TF, Tn, and sialosyl-Tn are located in immunologically privileged areas. The complex results obtained with anti-TF mAbs (after treatment of the tissue sections with sialidase from Vibrio cholerae) and the lectins amaranthin and jacalin revealed a differential distribution of the subtypes of sialosyl-TF [NeuAc alpha 2-3Gal beta 1-3GalNAc alpha 1-R and Gal beta 1-3 (NeuAc alpha 2-6)GalNAc alpha 1-R] in normal human tissues. From our data it can be inferred that TF, Tn, and sialosyl-Tn are promising targets for a cancer vaccine. |
| Databáze: | OpenAIRE |
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