Glial conversion of SVZ-derived committed neuronal precursors after ectopic grafting into the adult brain

Autor: Ralph Seidenfaden, Andreas Bosio, Angélique Desoeuvre, Isabelle Virard, Harold Cremer
Přispěvatelé: Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2006
Předmět:
Doublecortin Domain Proteins
animal diseases
Cell Separation
Mice
0302 clinical medicine
Tubulin
Lateral Ventricles
Neurons
0303 health sciences
Glutamate Decarboxylase
Stem Cells
Cell Differentiation
Cell sorting
Isoenzymes
Oligodendroglia
medicine.anatomical_structure
Microtubule-Associated Proteins
Neuroglia
Cell type
Doublecortin Protein
Interneuron
Cell Survival
Subventricular zone
Mice
Transgenic
Neural Cell Adhesion Molecule L1
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Biology
03 medical and health sciences
Cellular and Molecular Neuroscience
Interneurons
medicine
Animals
Brain Tissue Transplantation
Cell Lineage
Progenitor cell
Molecular Biology
030304 developmental biology
Neuropeptides
Cell Biology
Olfactory bulb
Mice
Inbred C57BL
Transplantation
Animals
Newborn
nervous system
Astrocytes
Forebrain
Sialic Acids
Neuroscience
Biomarkers
030217 neurology & neurosurgery
Stem Cell Transplantation
Zdroj: Molecular and Cellular Neuroscience
Molecular and Cellular Neuroscience, Elsevier, 2006, 32(1-2), pp.187-198
Molecular and Cellular Neuroscience, 2006, 32(1-2), pp.187-198
ISSN: 1044-7431
1095-9327
DOI: 10.1016/j.mcn.2006.04.003
Popis: In the adult mouse forebrain, large numbers of neuronal precursors, destined to become GABA- and dopamine-producing interneurons of the olfactory bulb (OB), are generated in the subventricular zone (SVZ). Although this neurogenic system represents a potential reservoir of stem and progenitor cells for brain repair approaches, information about the survival and differentiation of SVZ-derived cells in ectopic brain regions is still fragmentary. We show here that ectopic grafting of SVZ tissue gave rise to two morphologically distinguishable cell types displaying oligodendrocytic or astrocytic characteristics. Since SVZ tissue contains neuronal and glial progenitors, we used magnetic cell sorting to deplete A2B5+ glial progenitors from the dissociated SVZ and to positively select cells that express PSA-NCAM. This procedure allowed the purification of neuronal precursors expressing TUJ1, DCX and GAD65/67. Transplantation of these cells led again to the generation of the same two glial cell types, showing that committed interneuron precursors undergo glial differentiation outside their normal environment.
Databáze: OpenAIRE
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