The effects of aggregation and protein corona on the cellular internalization of iron oxide nanoparticles

Autor: Jean-François Berret, J. Courtois, Hélène Conjeaud, M. Seigneuret, Malak Safi
Přispěvatelé: Matière et Systèmes Complexes (MSC (UMR_7057)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2011
Předmět:
Light
Acrylic Resins
Iron oxide
Nanoparticle
Protein Corona
02 engineering and technology
Ferric Compounds
01 natural sciences
Nanomaterials
chemistry.chemical_compound
Scattering
Radiation
Citrates
Condensed Matter - Materials Science
endocytic compartment
Blood Proteins
021001 nanoscience & nanotechnology
Endocytosis
Membrane
Mechanics of Materials
Nanomedicine
0210 nano-technology
living cells
[PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft]
Iron oxide nanoparticles
Protein Binding
magnetic nanoparticles
Materials science
Biophysics
FOS: Physical sciences
Bioengineering
Nanotechnology
010402 general chemistry
Biomaterials
protein corona
Humans
Colloids
Protein Structure
Quaternary
flow cytometry
polymer coating
Materials Science (cond-mat.mtrl-sci)
Culture Media
0104 chemical sciences
Molecular Weight
internalization
Chemical engineering
chemistry
Hydrodynamics
Ceramics and Composites
Nanoparticles
Magnetic nanoparticles
Adsorption
Zdroj: Biomaterials
Biomaterials, Elsevier, 2011, 32 (35), pp.9353-9363. ⟨10.1016/j.biomaterials.2011.08.048⟩
ISSN: 0142-9612
Popis: Engineered inorganic nanoparticles are essential components in the development of nanotechnologies. For applications in nanomedicine, particles need to be functionalized to ensure a good dispersibility in biological fluids. In many cases however, functionalization is not sufficient : the particles become either coated by a corona of serum proteins or precipitate out of the solvent. In the present paper, we show that by changing the coating of iron oxide nanoparticles from a low-molecular weight ligand (citrate ions) to small carboxylated polymers (poly(acrylic acid)), the colloidal stability of the dispersion is improved and the adsorption/internalization of iron towards living mammalian cells is profoundly affected. Citrate-coated particles are shown to destabilize in all fetal-calf-serum based physiological conditions tested, whereas the polymer coated particles exhibit an outstanding dispersibility as well as a structure devoid of protein corona. The interactions between nanoparticles and human lymphoblastoid cells are investigated by transmission electron microscopy and flow cytometry. Two types of nanoparticle/cell interactions are underlined. Iron oxides are found either adsorbed on the cellular membranes, or internalized into membrane-bound endocytosis compartments. For the precipitating citrate-coated particles, the kinetics of interactions reveal a massive and rapid adsorption of iron oxide on the cell surfaces. The quantification of the partition between adsorbed and internalized iron was performed from the cytometry data. The results highlight the importance of resilient adsorbed nanomaterials at the cytoplasmic membrane.
Comment: 21 pages, 11 figures, accepted at Biomaterials (2011)
Databáze: OpenAIRE
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