Unveiling biogeographical patterns of the ichthyofauna in the Tuichi basin, a biodiversity hotspot in the Bolivian Amazon, using environmental DNA

Autor: Cédric Mariac, Fabrice Duponchelle, Guido Miranda, Camila Ramallo, Robert Wallace, Gabriel Tarifa, Carmen Garcia-Davila, Hernán Ortega, Julio Pinto, Jean-François Renno
Přispěvatelé: MARine Biodiversity Exploitation and Conservation (UMR MARBEC), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)
Jazyk: angličtina
Rok vydání: 2022
Předmět:
0106 biological sciences
Marine and Aquatic Sciences
Invasive Species
01 natural sciences
Data Management
0303 health sciences
Multidisciplinary
Geography
Ecology
Fishes
Eukaryota
Biodiversity
Freshwater Fish
Biogeography
Vertebrates
Medicine
Seasons
Brazil
Environmental Monitoring
Research Article
Freshwater Environments
Conservation of Natural Resources
Computer and Information Sciences
Ecological Metrics
Science
[SDE.MCG]Environmental Sciences/Global Changes
010603 evolutionary biology
03 medical and health sciences
Rivers
Species Colonization
Animals
DNA Barcoding
Taxonomic
14. Life underwater
Ecosystem
030304 developmental biology
Taxonomy
Ecology and Environmental Sciences
Organisms
Aquatic Environments
Biology and Life Sciences
Species Diversity
15. Life on land
Bodies of Water
DNA
Environmental
Lakes
Fish
Earth Sciences
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
Zoology
Zdroj: PLoS ONE
PLoS ONE, 2022, 17 (1), pp.e0262357. ⟨10.1371/journal.pone.0262357⟩
PLoS ONE, Vol 17, Iss 1 (2022)
Plos One (1932-6203) (Public Library of Science (PLoS)), 2022-01, Vol. 17, N. 1, P. e0262357 (25p.)
PLoS ONE, Vol 17, Iss 1, p e0262357 (2022)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0262357⟩
Popis: International audience; To date, more than 2400 valid fish species have been recorded in the Amazon basin. However, some regions remain poorly documented. This is the case in the Beni basin and in particular in one of its main sub-basins, the Tuichi, an Andean foothills rivers flowing through the Madidi National Park in the Bolivian Amazonia. The knowledge of its ichthyological diversity is, however, essential for the management and protection of aquatic ecosystems, which are threatened by the development of infrastructures (dams, factories and cities), mining and deforestation. Environmental DNA (eDNA) has been relatively little used so far in the Amazon basin. We sampled eDNA from water in 34 sites in lakes and rivers in the Beni basin including 22 sites in the Tuichi sub-basin, during the dry season. To assess the biogeographical patterns of the amazonian ichthyofauna, we implemented a metabarcoding approach using two pairs of specific primers designed and developed in our laboratory to amplify two partially overlapping CO1 fragments, one of 185bp and another of 285bp. We detected 252 fish taxa (207 at species level) among which 57 are newly identified for the Beni watershed. Species compositions are significantly different between lakes and rivers but also between rivers according to their hydrographic rank and altitude. Furthermore, the diversity patterns are related to the different hydro-ecoregions through which the Tuichi flows. The eDNA approach makes it possible to identify and complete the inventory of the ichthyofauna in this still poorly documented Amazon basin. However, taxonomic identification remains constrained by the lack of reference barcodes in public databases and does not allow the assignment of all OTUs. Our results can be taken into account in conservation and management strategies and could serve as a baseline for future studies, including on other Andean tributaries.
Databáze: OpenAIRE
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