Comparison of four nasal sampling methods for the detection of viral pathogens by RT-PCR-A GA(2)LEN project
| Autor: | Marek L. Kowalski, Marcin Kurowski, Ioannis Christodoulou, Karin Lodrup-Carlsen, Kai-Håkon Carlsen, Vegard Hovland, Lieke de Beer, Nikolaos G. Papadopoulos, Agnieszka Olszewska-Ziaber, Irini Spyridaki, Richard Molenkamp, Cornelis M. van Drunen |
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| Přispěvatelé: | AII - Amsterdam institute for Infection and Immunity, Ear, Nose and Throat, Medical Microbiology and Infection Prevention |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Nasal brush Adolescent viruses RT-PCR Visual Discomfort Nasal aspirate medicine.disease_cause Virus Article Nasal wash Nasal swab Young Adult Virology medicine Humans RNA Viruses Prospective Studies Child Respiratory Tract Infections Coronavirus Aged Aged 80 and over biology Reverse Transcriptase Polymerase Chain Reaction Common cold Middle Aged medicine.disease biology.organism_classification Upper respiratory infection Nasal Swab Parechovirus Enterovirus RNA Viral Female Rhinovirus Nasal Cavity |
| Zdroj: | Journal of Virological Methods Journal of virological methods, 156(1-2), 102-106. Elsevier |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/j.jviromet.2008.10.027 |
| Popis: | The aim of this study was to compare the efficacy and patient discomfort between four techniques for obtaining nasal secretions. Nasal secretions from 58 patients with symptoms of a common cold, from three clinical centers (Amsterdam, Lodz, Oslo), were obtained by four different methods: swab, aspirate, brush, and wash. In each patient all four sampling procedures were performed and patient discomfort was evaluated by a visual discomfort scale (scale 1-5) after each procedure. Single pathogen RT-PCRs for Rhinovirus (RV), Influenza virus and Adenovirus, and multiplex real-time PCR for RV, Enterovirus, Influenza virus, Adenovirus, Respiratory Syncytial Virus (RSV), Parainfluenza virus, Coronavirus, Metapneumovirus, Bocavirus and Parechovirus were performed in all samples. A specific viral cause of respiratory tract infection was determined in 48 patients (83%). In these, the detection rate for any virus was 88% (wash), 79% (aspirate), 77% (swab) and 74% (brush). The degree of discomfort reported was 2.54 for swabs, 2.63 for washes, 2.68 for aspirates and 3.61 for brushings. Nasal washes yielded the highest rate of viral detection without excessive patient discomfort. In contrast, nasal brushes produced the lowest detection rates and demonstrated the highest level of discomfort. |
| Databáze: | OpenAIRE |
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