The Immunoexpression of Heparanase 2 in Normal Epithelium, Intraepithelial, and Invasive Squamous Neoplasia of the Cervix

Autor: Sergio Mancini Nicolau, Adauto Castelo, Thérèse Rachell Theodoro, Renato Moretti Marques, Gustavo Rubino de Azevedo Focchi, Maria Aparecida da Silva Pinhal
Přispěvatelé: Pinhal, Maria Aparecida https://orcid.org/0000-0003-4001-1663, FOCCHI, GUSTAVO https://orcid.org/0000-0002-5978-0879, Pinhal, Maria Aparecida/J-1797-2012
Rok vydání: 2012
Předmět:
Pathology
Uterine Cervical Neoplasms
Expression
Endometrium
Metastasis
Reference Values
Prostate
Glucuronidase
Cervical cancer
medicine.diagnostic_test
Breast-Cancer Patients
Biopsy
Needle
Obstetrics & Gynecology
Obstetrics and Gynecology
General Medicine
Middle Aged
Prognosis
Immunohistochemistry
Squamous intraepithelial lesion
Cell Transformation
Neoplastic
medicine.anatomical_structure
Invasive Squamous Cell Carcinoma
Carcinoma
Squamous Cell
Female
Adult
medicine.medical_specialty
Cells
Heparanase 2
Cervical intraepithelial neoplasia
Biopsy
Biomarkers
Tumor
medicine
Carcinoma
Humans
Neoplasm Invasiveness
Cervical Intraepithelial Neoplasia
Cervix
Aged
Neoplasm Staging
Retrospective Studies
business.industry
Uterine Cervical Dysplasia
medicine.disease
Patient Survival
Sulfate
Pancreatic-Cancer
ROC Curve
Localization
Case-Control Studies
Tumor Progression
business
Zdroj: Web of Science
Repositório Institucional da UNITAU
Universidade de Taubaté (UNITAU)
instacron:UNITAU
ISSN: 1089-2591
DOI: 10.1097/lgt.0b013e3182422c69
Popis: Made available in DSpace on 2019-09-12T16:53:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2012 Objective. Heparanase 2 (HPSE2) is expressed in various tissues, including the brain, intestine, prostate, breast, and endometrium. The aim of this study was to investigate the role of HPSE2 in cervical carcinogenesis, which has not been clarified to date. Materials and Methods. The immunoexpression of HPSE2 in normal and neoplastic cervical squamous epithelia was determined using a semiquantitative (SQ) method and an index of expression (IE) method, using Image Lab Software. A total of 230 cervical tissue samples were analyzed and segregated into the following diagnostic groups: normal (27.4%), cervical intraepithelial neoplasia 1 (CIN 1, 15.2%), CIN 2 (16.5%), CIN 3(15.2%), and invasive neoplasia (25.7%). The mean HPSE2 expression in the normal group was significantly lower than that of the other groups individually or combined (p < .001, for all combinations). The immunoexpression via the SQ method was significantly greater in the CIN 3 group compared with that in the CIN 1 group (p = .02). The mean immunoexpression of the high-grade squamous intraepithelial lesion groups was significantly greater than those of the normal and low-grade squamous intraepithelial lesion groups (p < .001) and lower compared with that of the invasive neoplasia group (p < .001). There were no statistically significant differences in the immunoexpression of HPSE2 among the different clinical states within the invasive neoplasia group. Conclusions. The SQ method produced a greater sensitivity and specificity than did the index of expression method. There was a progressive increase in the mean HPSE2 immunoexpression according to the severity of the cervical lesion from the low-grade squamous intraepithelial lesion group to the invasive neoplasm group, whereas the normal group displayed the lowest level of expression. This is a novel study concerning HPSE2 in the cervix and cervical cancer carcinogenesis. [Marques, Renato Moretti; Nicolau, Sergio Mancini] Univ Fed Sao Paulo, Dept Gynecol, Div Gynecol Oncol, Paulista Sch Med Prof, BR-04038032 Sao Paulo, Brazil [Marques, Renato Moretti] Universidade de Taubaté (Unitau), Dept Obstet & Gynecol [Focchi, Gustavo Rubino] Univ Fed Sao Paulo, Dept Pathol, Paulista Sch Med, BR-04038032 Sao Paulo, Brazil [Theodoro, Therese Rachell; Pinhal, Maria Aparecida] ABC, Coll Med, Dept Biochem, Sao Paulo, Brazil [Castelo, Adauto] Univ Fed Sao Paulo, Dept Med, Div Infect Dis, Paulista Sch Med, BR-04038032 Sao Paulo, Brazil
Databáze: OpenAIRE
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