Ex Vivo Model in Cholestasis Research
| Autor: | Karsten, Ruby E H, Oosterhuis, Dorenda, van Wijk, Louise A, Olinga, Peter, Vinken, Mathieu |
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| Přispěvatelé: | Pharmaceutical Analysis, Pharmaceutical Technology and Biopharmacy, Biopharmaceuticals, Discovery, Design and Delivery (BDDD), Groningen Institute for Organ Transplantation (GIOT) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Experimental Cholestasis Research, 351-362 STARTPAGE=351;ENDPAGE=362;TITLE=Experimental Cholestasis Research Experimental Cholestasis Research Methods in Molecular Biology ISBN: 9781493994199 Methods in Molecular Biology Methods in Molecular Biology-Experimental Cholestasis Research |
| ISSN: | 1064-3745 1940-6029 |
| Popis: | To mimic (human) cholestasis in vitro requires multiple triggers to establish a diseased phenotype. However, this is currently not simulated by existing in vitro models. Therefore, there is a high need for multicellular systems similar to the human physiology. In such an in vitro model, cell-cell interactions and intact bile canaliculi with functional bile flow should be present and preserved during long-term culture. Precision-cut liver slices represent an ex vivo tissue culture technique that replicates most of the multicellular characteristics of a whole liver in vivo. This chapter describes the preparation and culturing of (human) precision-cut liver slices. Furthermore, a protocol to use the precision-cut liver slices technique to predict drug-induced cholestatic liver injury is described. |
| Databáze: | OpenAIRE |
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