Evidence that the caterpillar salivary enzyme glucose oxidase provides herbivore offense in solanaceous plants
| Autor: | Gary W. Felton, Judith K. Brown, Richard O. Musser, Don Cipollini, Spencer A. Williams, Sue M. Hum-Musser |
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| Rok vydání: | 2005 |
| Předmět: |
Nicotine
Saliva Physiology Nicotiana tabacum Trypsin inhibitor Biochemistry Lycopersicon Glucose Oxidase chemistry.chemical_compound Tobacco medicine Animals Solanaceae Plant Diseases biology fungi food and beverages General Medicine biology.organism_classification Elicitor Lepidoptera chemistry Larva Insect Science Trypsin Inhibitors Systemic acquired resistance Salicylic acid medicine.drug |
| Zdroj: | Archives of Insect Biochemistry and Physiology. 58:128-137 |
| ISSN: | 1520-6327 0739-4462 |
| Popis: | The insect salivary enzyme glucose oxidase (GOX) can inhibit wound-inducible nicotine production in tobacco, Nicotiana tabacum. We examined whether salivary gland extracts of Helicoverpa zea lacking active GOX could still suppress nicotine in tobacco, Nicotiana tabacum, and whether GOX could suppress wound-inducible defenses of another Solanaceous plant, tomato Lycopersicon esculentum. Tobacco leaves were wounded with a cork borer and treated with water, salivary gland extracts with active GOX (SxG), or salivary gland extracts with inactive GOX (SxI). After three days, leaves treated with SxG had significantly less nicotine than all other wounded treatments. Neonates that fed on the terminal leaves of tobacco plants treated with SxG had significantly higher survival than neonates that fed on leaves treated with either SxI or water. This evidence supports the assertion that GOX is the salivary factor responsible for the suppression of tobacco plant nicotine production by H. zea saliva. Results for the NahG tobacco plants, which lack salicylic acid (SA) due to a transgene for bacterial SA hydroxylase, indicate that suppression of nicotine by GOX does not require SA. However, tobacco leaves that were wounded and treated with SxG had significantly higher levels of the SA-mediated PR-1a protein than leaves treated with SxI or water. Leaves of tomato plants wounded with scissors and then treated with SxG had trypsin inhibitor levels that were moderately lower than plants wounded and treated with purified GOX, water, or SxI. However, all the wounded tomato leaves irrespective of treatment resulted in lower caterpillar growth rates than the non-wounded tomato leaves. Glucose oxidase is the first insect salivary enzyme shown to suppress wound-inducible herbivore defenses of plants. |
| Databáze: | OpenAIRE |
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