Immunophenotyping of T cells in the peripheral circulation in psoriasis
| Autor: | Laxmisha Chandrashekar, S. Indhumathi, M. Priyadarssini, D Divya Priya, Devinder Mohan Thappa, Medha Rajappa |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
CD4-Positive T-Lymphocytes Male Pathology medicine.medical_treatment Clinical Biochemistry T-Lymphocytes Regulatory 030207 dermatology & venereal diseases 0302 clinical medicine Immunophenotyping T-Lymphocyte Subsets Immunology and Allergy education.field_of_study medicine.diagnostic_test Middle Aged Flow Cytometry Infectious Diseases medicine.anatomical_structure Cytokine Cytokines Female medicine.symptom Keratinocyte Microbiology (medical) Adult medicine.medical_specialty Immunology Population Inflammation Microbiology Peripheral blood mononuclear cell Flow cytometry 03 medical and health sciences Th2 Cells Psoriasis medicine Humans education business.industry Biochemistry (medical) Th1 Cells medicine.disease 030104 developmental biology Cross-Sectional Studies Leukocytes Mononuclear Th17 Cells business |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0967-4845 |
| Popis: | Psoriasis is a T-helper (Th)-1/Th17-mediated chronic inflammatory disease. Cytokine mediated interaction between T lymphocytes and keratinocytes lead to keratinocyte hyper-proliferation, which leads to further inflammation in the psoriatic plaques. There is an increased population of T-helper cells in the skin lesions as well as in the peripheral circulation in psoriasis. However, the relative percentage of each T-cell phenotype in the disease pathogenesis is understudied. Our aim was to study the immune-phenotype of the different T-helper/T-reg cell subsets in patients with psoriasis, with respect to healthy controls.A total of 189 cases of psoriasis and 189 age- and gender-matched healthy controls were recruited in this cross-sectional study. Disease severity was determined by psoriasis area severity index (PASI) scoring. Peripheral blood mononuclear cells were isolated by Ficoll-Paque density centrifugation, and T-cell immunophenotyping was done by flow cytometric analysis.In psoriasis, we observed an imbalance in T-cell immunophenotype, characterised by an increase in Th1/Th17 cells and a relative decrease in Th2/T-reg cells, as compared to the healthy controls. We also found that the percentage of Th1/Th17 cells showed a linear trend, increasing with increasing disease severity (PASI).Our results suggest an immune-dysregulation in psoriasis associated with a predominance of Th1/Th17 phenotype, especially with increasing severity of the disease. |
| Databáze: | OpenAIRE |
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