Functional analysis of Ultrabithorax in the wing-dimorphic planthopper Nilaparvata lugens (Stål, 1854) (Hemiptera: Delphacidae)
Autor: | Hao-Hao Chen, Yi-Lai Liu, Hai-Jun Xu, Jin-Li Zhang, Sheng-Jie Fu, Sun-Jie Chen |
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Rok vydání: | 2020 |
Předmět: |
Male
0301 basic medicine animal structures Biology Hemiptera 03 medical and health sciences Planthopper 0302 clinical medicine RNA interference Genetics Animals Wings Animal Amino Acid Sequence Cloning Molecular Hox gene Ultrabithorax Appendage General Medicine biology.organism_classification 030104 developmental biology Gene Knockdown Techniques 030220 oncology & carcinogenesis Insect Proteins Female Delphacidae Homeotic gene Sequence Alignment |
Zdroj: | Gene. 737:144446 |
ISSN: | 0378-1119 |
Popis: | The homeotic complex (Hox) gene Ultrabithorax (Ubx) plays pivotal roles in modifying specific morphological differences among the second (T2), the third thoracic (T3), and the first abdomen (A1) segment in several insects. Whether Ubx regulates wing dimorphism and other morphological traits in the delphacid family (order Hemiptera) remains elusive. In this study, we cloned a full-length Ubx ortholog (NlUbx) from the wing-dimorphic planthopper Nilaparvata lugens, and identified two NlUbx isoforms. RNA-interference (RNAi)-mediated silencing of NlUbx in short-winged BPH nymphs significantly induced the development of wing-like appendages from T3 wingbuds, and this effect is likely mediated by the insulin/insulin-like signaling pathway. RNAi knockdown of NlUbx in long-winged BPH nymphs led to a transformation from hindwings to forewings. Additionally, silencing of NlUbx not only dramatically changed the T3 morphology, but also led to jumping defect of T3 legs. First-instar nymphs derived from parental RNAi had an additional leg-like appendages on A1. These results suggest that Ubx plays a role in determining some morphological traits in delphacid planthoppers, and thus help in understanding evolution of morphological characteristics in arthropods. |
Databáze: | OpenAIRE |
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