Sequences in the PreC Region of Duck Hepatitis B Virus Affect Pregonomic RNA Accumulation
| Autor: | Don Ganem, Russell C. Hirsch, Christopher J. Chang |
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| Rok vydání: | 1995 |
| Předmět: |
Messenger RNA
Base Sequence Transcription Genetic biology Polyadenylation Molecular Sequence Data Duck hepatitis B virus RNA-dependent RNA polymerase RNA Genome Viral biology.organism_classification Molecular biology Hepatitis Virus Duck Reverse transcriptase Cell Line Phenotype Transcription (biology) Virology DNA Viral Mutagenesis Site-Directed biology.protein Animals RNA Viral Polymerase Sequence Deletion |
| Zdroj: | Virology. 207:549-554 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.1995.1115 |
| Popis: | The pregenomic RNA of hepadnaviruses serves as both the mRNA for the core and polymerase proteins and the RNA template for reverse transcription. We have identified a region in the duck hepatitis B virus pregenomic RNA transcription unit that is critical for the accumulation of this transcript. This 85-nt region, termed alpha, is located within the preC region; deletion of alpha results in drastically reduced steady-state levels of pregenomic RNA. This effect is not due to reduction in transcription initiation or to enhancement of premature polyadenylation at the 5' copy of the viral poly(A) signal. However, this phenotype is suppressed by deletion of a second, larger region (beta) located ca. 1 kb downstream. The activity of the alpha element is tissue- and species-nonspecific; however, it displays absolute orientation-dependence and its activity is influenced by its position within the transcript. Models for its action are discussed. |
| Databáze: | OpenAIRE |
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