Strong seed-specific protein expression from the Vigna radiata storage protein 8SGα promoter in transgenic Arabidopsis seeds
| Autor: | Mee-Len Chye, Mo-Xian Chen, Jie-Sheng Liu, Chao Xu, Shu-Xiao Zheng, Wei-Dong Yang, Yue-Ning Yang, Hong-Ye Li |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Recombinant Fusion Proteins
Molecular Sequence Data Arabidopsis Bioengineering Beta-glucuronidase Genes Plant Applied Microbiology and Biotechnology Vigna Chromosome Walking Gene Expression Regulation Plant Botany Storage protein Arabidopsis thaliana Promoter Regions Genetic Gene Glucuronidase chemistry.chemical_classification Base Sequence biology Seed Storage Proteins fungi food and beverages Fabaceae General Medicine Plants Genetically Modified biology.organism_classification Molecular biology Transformation (genetics) chemistry Seeds Cauliflower mosaic virus Sequence Alignment Genome Plant Biotechnology |
| Zdroj: | Journal of Biotechnology. 174:49-56 |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/j.jbiotec.2014.01.027 |
| Popis: | Vigna radiata (mung bean) is an important crop plant and is a major protein source in developing countries. Mung bean 8S globulins constitute nearly 90% of total seed storage protein and consist of three subunits designated as 8SGα, 8SGα' and 8SGβ. The 5'-flanking sequences of 8SGα' has been reported to confer high expression in transgenic Arabidopsis seeds. In this study, a 472-bp 5'-flanking sequence of 8SGα was identified by genome walking. Computational analysis subsequently revealed the presence of numerous putative seed-specific cis-elements within. The 8SGα promoter was then fused to the gene encoding β-glucuronidase (GUS) to create a reporter construct for Arabidopsis thaliana transformation. The spatial and temporal expression of 8SGα∷GUS, as investigated using GUS histochemical assays, showed GUS expression exclusively in transgenic Arabidopsis seeds. Quantitative GUS assays revealed that the 8SGα promoter showed 2- to 4-fold higher activity than the Cauliflower Mosaic Virus (CaMV) 35S promoter. This study has identified a seed-specific promoter of high promoter strength, which is potentially useful for directing foreign protein expression in seed bioreactors. |
| Databáze: | OpenAIRE |
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