The balance of the immune system between HLA-G and NK cells in unexplained recurrent spontaneous abortion and polymorphisms analysis
| Autor: | Fateme Arjmand, Seyed Ali Mirghanizadeh, Nasrin Ghasemi, Morteza Samadi |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Linkage disequilibrium Genotype Immunology DNA Mutational Analysis Single-nucleotide polymorphism Human leukocyte antigen Biology Polymorphism Single Nucleotide Linkage Disequilibrium 03 medical and health sciences Young Adult 0302 clinical medicine Gene Frequency Pregnancy Recurrence HLA-G Humans Genetic Predisposition to Disease Allele Promoter Regions Genetic Allele frequency Genetic Association Studies Genetics HLA-G Antigens Abortion Spontaneous Killer Cells Natural 030104 developmental biology Population study Female 030215 immunology |
| Zdroj: | Immunologic research. 64(3) |
| ISSN: | 1559-0755 |
| Popis: | Human leukocyte antigen (HLA)-G is involved in immunoregulatory processes and particularly in pathogenesis of inflammatory disorders such as recurrent spontaneous abortions (RSA). The purpose of the current study was to examine whether two single nucleotide polymorphisms (SNPs) of HLA-G gene (rs1736936 and HLA-G*0105N) influence susceptibility to recurrent spontaneous abortion. Genomic DNA from 117 RSA patients and 117 normal fertile control individuals was isolated using the salted out method. The two single nucleotide polymorphisms in HLA-G gene were analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Differences between the two groups were analyzed by SPSS19 software using Chi-square test. The results revealed a significant increase in HLA-G*0105N allele in the proportion of whole group of RSA women compared with fertile controls (P value = 0.015), OR (95 % CI) = 2.054 (1.798-2.347), as well as an absence of homozygosity for HLA-G*0105N in the study population. No significant difference was observed between the RSA and the fertile groups in terms of alleles and genotypes frequency of rs1736936 (P value = 0.323), OR (95 CI %) = 1.056 (0.844-1.319). The presented data suggest that the investigated HLA-G*0105N allele is potentially associated with RSA through linkage disequilibrium with other genetic elements. Meanwhile, the rs1736936 SNP do not predispose to RSA in the study population. |
| Databáze: | OpenAIRE |
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