Persistence ofBacillus thuringiensisbioinsecticides in the gut of human-flora-associated rats
| Autor: | Tine Rask Licht, Andrea Wilcks, Niels Bohse Hendriksen, Bjarne Munk Hansen |
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| Rok vydání: | 2006 |
| Předmět: |
Microbiology (medical)
Immunology Bacillus thuringiensis Enterotoxin Microbiology Rats Sprague-Dawley Feces Animals Humans Immunology and Allergy Pest Control Biological Electrophoresis Agar Gel Spores Bacterial Gastrointestinal tract biology fungi General Medicine biology.organism_classification Animal Feed Bacillales Rats Spore Gastrointestinal Tract Infectious Diseases Bacteria Temperature gradient gel electrophoresis |
| Zdroj: | Technical University of Denmark Orbit |
| ISSN: | 1574-695X 0928-8244 |
| DOI: | 10.1111/j.1574-695x.2006.00169.x |
| Popis: | The capability of two bioinsecticide strains of Bacillus thuringiensis (ssp. israelensis and ssp. kurstaki) to germinate and persist in vivo in the gastrointestinal tract of human-flora-associated rats was studied. Rats were dosed either with vegetative cells or spores of the bacteria for 4 consecutive days. In animals fed spores, B. thuringiensis cells were detected in faecal and intestinal samples of all animals, whereas vegetative cells only poorly survived the gastric passage. Heat-treatment of intestinal samples, which kills vegetative cells, revealed that B. thuringiensis spores were capable of germination in the gastrointestinal tract. In one animal fed spores of B. thuringiensis ssp. kurstaki, these bacteria were detected at high density (10(3)-10(4) CFU g(-1) faecal and intestinal samples) even 2 weeks after the last dosage. In the same animal, passage of B. thuringiensis ssp. kurstaki to the spleen was observed; however, no other adverse effects were observed. Denaturing gradient gel electrophoresis of PCR-amplified bacterial 16S rRNA genes in faecal samples revealed no major effect of B. thuringiensis on the composition of the indigenous gut bacteria. Additionally, no cytotoxic effect was detectable in gut samples by Vero cell assay. |
| Databáze: | OpenAIRE |
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