Microquantitative analysis of the intra-acinar profiles of glutamate dehydrogenase in rat liver
| Autor: | D. Sasse, I P Maly |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
medicine.medical_specialty Histology Adult male Glutamine synthesis Biology Glutamate Dehydrogenase Internal medicine Liver Acinus medicine Animals chemistry.chemical_classification Glutamate dehydrogenase Rats Inbred Strains NAD Rats Kinetics Endocrinology Enzyme Liver chemistry Rat liver Immunohistochemistry Female NAD+ kinase Anatomy NADP |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 39:1121-1124 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1177/39.8.1856459 |
| Popis: | In adult male and female rat liver, the activity of NAD(+)-and NADP(+)-dependent glutamate dehydrogenase (GDH) was microquantitatively measured in tissue samples of 50-150 ng, microdissected continuously along the sinusoidal length. Total activity of GDH with NAD+ as co-factor was found to be higher by a ratio of about 1:2.3 than with NADP+. All intra-acinar enzyme profiles, irrespective of sex, showed an increasing gradient of GDH activity from the periportal beginning to the perivenous end. These findings are at variance with the immunohistochemical localization of GDH in rat liver. The microquantitative GDH profiles with higher perivenous values could indicate a more pronounced glutamine synthesis in Zone 3 of the liver acinus. |
| Databáze: | OpenAIRE |
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