Ca2+ release and Ca2+ influx in Chinese hamster ovary cells expressing the cloned mouse B2 bradykinin receptor: tyrosine kinase inhibitor-sensitive and -insensitive processes
| Autor: | Haruhiro Higashida, Yasuhiro Kimura, Shigeru Yokoyama, Megumi Taketo |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Thapsigargin
Receptor Bradykinin B2 CHO Cells Inositol 1 4 5-Trisphosphate Biology Transfection Bradykinin chemistry.chemical_compound Cricetinae Second messenger Cyclic AMP Animals Homeostasis Cloning Molecular Enzyme Inhibitors Phosphorylation Phosphotyrosine Molecular Biology Manganese CHO cell Binding Sites B2 receptor Phospholipase C Chinese hamster ovary cell Receptors Bradykinin Inositol trisphosphate Cell Biology Protein-Tyrosine Kinases Molecular biology Genistein Isoflavones chemistry Calcium ion homeostasis Second messenger system Calcium Heterologous expression B2 Bradykinin Receptor Intracellular |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1355(1):89-98 |
| ISSN: | 0167-4889 |
| DOI: | 10.1016/s0167-4889(96)00126-7 |
| Popis: | A cDNA encoding a mouse B 2 bradykinin (BK) receptor was stably transfected in Chinese hamster ovary (CHO) cells. In two resulting transformants, mouse B 2 BK receptor was found to induce a twofold elevation in the inositol-1,4,5-trisphosphate level. In a pertussis toxin-insensitive manner, BK also produced a biphasic increase in the intracellular Ca 2+ concentration ([Ca 2+ ] i ). The initial elevation in [Ca 2+ ] i was abolished by thapsigargin pretreatment in Ca 2+ -free medium. The second phase was dependent on external Ca 2+ . The BK/inositol trisphosphate- and thapsigargin-sensitive Ca 2+ stores required extracellular Ca 2+ for refilling. Ca 2+ influx induced by BK and thapsigargin was confirmed by Mn 2+ entry through Ca 2+ influx pathways producing Mn 2+ quenching. Genistein, a tyrosine kinase inhibitor, partially decreased the BK-induced [Ca 2+ ] i increase during the sustained phase and the rate of Mn 2+ entry. BK had essentially no effect on the intracellular cyclic AMP level. The results suggest that the mouse B 2 BK receptor couples to phospholipase C in CHO cells and that its activation results in biphasic [Ca 2+ ] i increases, by mobilization of intracellular Ca 2+ and store-depletion-mediated Ca 2+ influx, the latter of which is tyrosine phosphorylation-dependent. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect