Studies on the in vitro transcription and translation of the lac operon
| Autor: | Beatrice P. Chen, Herbert Weissbach, Carlos Spears, Nathan Brot, Hsiang-Fu Kung |
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| Rok vydání: | 1974 |
| Předmět: |
Time Factors
Transcription Genetic Uracil Nucleotides Biophysics lac operon RNA polymerase II Lac repressor Biochemistry Polyethylene Glycols trp operon chemistry.chemical_compound Ribosomal protein Transcription (biology) RNA polymerase Operon Cyclic AMP Escherichia coli Carbon Radioisotopes RNA Messenger Molecular Biology biology General transcription factor Molecular biology Guanine Nucleotides Galactosidases Kinetics RNA Bacterial chemistry Spectrophotometry Protein Biosynthesis Mutation biology.protein bacteria Rifampin Ribosomes |
| Zdroj: | Archives of Biochemistry and Biophysics. 160:168-174 |
| ISSN: | 0003-9861 |
| Popis: | With the use of rifampicin to stop the initiation of RNA synthesis, the in vitro synthesis of β -galactosidase has been carried out in two separate stages, transcription and translation. In this system it has been possible to examine whether substances, which affect the synthesis of the enzyme, function at the transcription or translation level of protein synthesis. Guanosine 5′-diphosphate 2′ (or 3′) diphosphate and 3′5′ cyclic AMP, affect mainly the transcription of the lac operon, whereas polyethyleneglycol 6000 stimulates the translation of lac mRNA or protects the mRNA. It appears that unknown factors present in the 1 m NH 4 Cl ribosomal wash are essential for transcription of the lac operon even in the presence of saturating levels of RNA polymerase. Both the S-200 fraction and washed ribosomes stimulate transcription of the lac operon as do 30S and 50S ribosomal subunits. The effect of the 50S particles can be replaced by the 50S ribosomal proteins, L 7 and L 12 . |
| Databáze: | OpenAIRE |
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