Assimilating cell sheets and hybrid scaffolds for dermal tissue engineering

Autor: Thiam Chye Lim, Kee Woei Ng, Wanrong Tham, Dietmar W. Hutmacher
Rok vydání: 2005
Předmět:
Keratinocytes
Materials science
Cell Survival
Polymers
Immunocytochemistry
Biomedical Engineering
Cell Culture Techniques
Biocompatible Materials
skin
extracellular matrix
poly(lactic-co-glycolic acid)
collagen
tissue engineering
law.invention
Biomaterials
Extracellular matrix
chemistry.chemical_compound
Tissue engineering
Polylactic Acid-Polyglycolic Acid Copolymer
law
Materials Testing
Animals
Humans
Lactic Acid
Hyaluronic Acid
Growth Substances
Cells
Cultured
Cell Proliferation
chemistry.chemical_classification
Tissue Engineering
Cell growth
Metals and Alloys
DNA
Dermis
Fibroblasts
090300 BIOMEDICAL ENGINEERING
111600 MEDICAL PHYSIOLOGY
Actins
Coculture Techniques
Cell biology
Extracellular Matrix
Vascular endothelial growth factor
Enzyme
chemistry
Ceramics and Composites
069900 OTHER BIOLOGICAL SCIENCES
Keratinocyte growth factor
Collagen
Electron microscope
Polyglycolic Acid
Biomedical engineering
Zdroj: Journal of Biomedical Materials Research Part A
ISSN: 1549-3296
Popis: Cell sheets can be used to produce neo-tissue with mature extracellular matrix. However, extensive contraction of cell sheets remains a problem. We devised a technique to overcome this problem and applied it to tissue engineer a dermal construct. Human dermal fibroblasts were cultured with poly(lactic-co-glycolic acid)-collagen meshes and collagen-hyaluronic acid foams. Resulting cell sheets were folded over the scaffolds to form dermal constructs. Human keratinocytes were cultured on these dermal constructs to assess their ability to support bilayered skin regeneration. Dermal constructs produced with collagen-hyaluronic acid foams showed minimal contraction, while those with poly(lactic-co-glycolic acid)-collagen meshes curled up. Cell proliferation and metabolic activity profiles were characterized with PicoGreen and AlamarBlue assays, respectively. Fluorescent labeling showed high cell viability and F-actin expression within the constructs. Collagen deposition was detected by immunocytochemistry and electron microscopy. Transforming Growth Factor-alpha and beta1, Keratinocyte Growth Factor and Vascular Endothelial Growth Factor were produced at various stages of culture, measured by RT-PCR and ELISA. These results indicated that assimilating cell sheets with mechanically stable scaffolds could produce viable dermal-like constructs that do not contract. Repeated enzymatic treatment cycles for cell expansion is unnecessary, while the issue of poor cell seeding efficiency in scaffolds is eliminated.
Databáze: OpenAIRE
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