The use of high-hydrostatic pressure treatment to decellularize blood vessels
| Autor: | Akio Kishida, Soichiro Kitamura, Kwangwoo Nam, Ayako Murakoshi, Yoshihide Hashimoto, Seiichi Funamoto, Kazuo Niwaya, Tsuyoshi Kimura, Toshiya Fujisato |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Decellularization
Materials science Swine Hydrostatic pressure Ice Transplantation Heterologous Biophysics Bioengineering CELL DEBRIS medicine.disease Biomaterials Cellular infiltration Transplantation Blood pressure medicine.anatomical_structure Mechanics of Materials In vivo Ceramics and Composites medicine Hydrostatic Pressure Animals Blood Vessels Biomedical engineering Blood vessel |
| Zdroj: | Biomaterials. 31(13) |
| ISSN: | 1878-5905 |
| Popis: | A decellularization method using high-hydrostatic pressure (HHP) technology (>600MPa) is described. The HHP disrupts the cells inside the tissue. The cell debris can be eliminated with a simple washing process, producing clean, decellularized tissue. In this study, porcine aortic blood vessel was decellularized by HHP. The mechanical properties and in vivo performance of the decellularized tissue were evaluated. Mechanical properties of the decellularized tissue were not altered by the HHP treatment. Reduced inflammation of the decellularized tissue was confirmed by xenogenic transplant experimentation. An allogenic transplantation study showed that decellularized blood vessel endured the arterial blood pressure, and there was no clot formation on the luminal surface. In addition, cellular infiltration into the vessel wall was observed 4 weeks after implantation, suggesting that HHP treatments could be applied widely as a high-quality decellularization method. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect