Antimicrobial Properties of Palladium and Platinum Nanoparticles: A New Tool for Combating Food-Borne Pathogens

Autor: Petr Slepička, Dominik Fajstavr, Ondrej Chlumsky, Jitka Viktorova, Hana Sykorova, Katerina Demnerova, Hana Michova, Sabina Purkrtova, Pavel Ulbrich
Jazyk: angličtina
Rok vydání: 2021
Předmět:
antimicrobial properties
Metal Nanoparticles
02 engineering and technology
medicine.disease_cause
Kidney
Bacterial cell structure
Foodborne Diseases
minimum inhibitory concentrations
food-borne pathogens
palladium nanoparticles
Biology (General)
Cytotoxicity
Spectroscopy
Cells
Cultured
0303 health sciences
biology
Chemistry
General Medicine
021001 nanoscience & nanotechnology
Antimicrobial
acute cytotoxicity
Computer Science Applications
Anti-Bacterial Agents
Salmonella enterica
0210 nano-technology
Palladium
QH301-705.5
Catalysis
Article
Microbiology
Inorganic Chemistry
03 medical and health sciences
Listeria monocytogenes
medicine
Humans
Physical and Theoretical Chemistry
Molecular Biology
Escherichia coli
QD1-999
030304 developmental biology
Platinum
Bacteria
Organic Chemistry
Biofilm
Fibroblasts
biology.organism_classification
HaCaT
Food Microbiology
platinum nanoparticles
Zdroj: International Journal of Molecular Sciences, Vol 22, Iss 7892, p 7892 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 15
ISSN: 1661-6596
1422-0067
Popis: Although some metallic nanoparticles (NPs) are commonly used in the food processing plants as nanomaterials for food packaging, or as coatings on the food handling equipment, little is known about antimicrobial properties of palladium (PdNPs) and platinum (PtNPs) nanoparticles and their potential use in the food industry. In this study, common food-borne pathogens Salmonella enterica Infantis, Escherichia coli, Listeria monocytogenes and Staphylococcus aureus were tested. Both NPs reduced viable cells with the log10 CFU reduction of 0.3–2.4 (PdNPs) and 0.8–2.0 (PtNPs), average inhibitory rates of 55.2–99% for PdNPs and of 83.8–99% for PtNPs. However, both NPs seemed to be less effective for biofilm formation and its reduction. The most effective concentrations were evaluated to be 22.25–44.5 mg/L for PdNPs and 50.5–101 mg/L for PtNPs. Furthermore, the interactions of tested NPs with bacterial cell were visualized by transmission electron microscopy (TEM). TEM visualization confirmed that NPs entered bacteria and caused direct damage of the cell walls, which resulted in bacterial disruption. The in vitro cytotoxicity of individual NPs was determined in primary human renal tubular epithelial cells (HRTECs), human keratinocytes (HaCat), human dermal fibroblasts (HDFs), human epithelial kidney cells (HEK 293), and primary human coronary artery endothelial cells (HCAECs). Due to their antimicrobial properties on bacterial cells and no acute cytotoxicity, both types of NPs could potentially fight food-borne pathogens.
Databáze: OpenAIRE
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